Human Decorin / DCN ELISA Kit PicoKine™

Human Decorin / DCN ELISA Kit PicoKine™ (96 Tests). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0749
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Decorin / DCN ELISA Kit PicoKine™

See all DCN products

SKU/Catalog Number

EK0749

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Decorin / DCN ELISA Kit PicoKine™ (96 Tests). Quantitate Human DCN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Decorin / DCN ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0749)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: sf21; Immunogen sequence: G17-K359

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0749 is reactive to DCN in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0749 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0749-CAP Anti-Human DCN Pre-coated 96-well strip microplate 1
EK0749-ST Human DCN Standard 2 vials, 10 ng/tube
EK0749-DA Human DCN Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0010.0630.1290.3220.6201.1241.7972.259

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect DCN, Dilution ratio of 1:1, concentration in serum is around 2ng/ml.

Some articles we found to cite concentrations of DCN in samples: 23026977, 23607294 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Decorin / DCN ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)602471001562701067
Standard deviation4.5616.5470.074.3120.2588.56
CV (%)7.6%6.7%7%7.7%7.5%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of DCN in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 160515059554.528.2%
Sample 224727930228727820.17.2%
Sample 3100196292297696528.612.9%
*number of samples for each test n=16.

Gene/Protein Information For DCN (Source: Uniprot.Org, NCBI)

Uniprot ID

P07585

Gene ID

1634

Gene Name

DCN

Full Name

Decorin

Weight

39.747kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B DCN CSCD, DSPG2, PG40, PGII, PGS2, SLRR1B decorin decorin|bone proteoglycan II|dermatan sulphate proteoglycans II|proteoglycan core protein|small leucine-rich protein 1B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on DCN, check out the DCN Infographic

DCN infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for DCN: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK0749

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human Decorin / DCN ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human Decorin / DCN ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

23 Customer Q&As for Human Decorin / DCN ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-08-03

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-08-03

Question

Q: Can DCN ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

E. Tiwari

Verified customer

Asked: 2020-07-20

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-07-20

Question

Q: which procedure should I follow in order to thaw whole blood sample for DCN ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DCN for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-01-20

Question

Has EK0749 been validated to detect the protein in its native form (if it has glycosaminoglycan chains on it)?

Verified customer

Asked: 2020-01-17

Answer

The Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) has been validated to detect the protein in its native form. However, our lab hasn't validated if it has glycosaminoglycan chains on it.

Boster Scientific Support

Answered: 2020-01-19

Question

Is the EK0749 kit designed for XYZ species or is it designed for a different species such as human and cross reacts with XYZ species?

Verified customer

Asked: 2020-01-08

Answer

The reactivity for Human Decorin / DCN ELISA Kit PicoKine EK0749 is human and no detectable cross reactivity.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the Uniprot/Accession number EK0749 is designed for?

Verified customer

Asked: 2020-01-07

Answer

For the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749), Uniprot/Accession number is P07585.

Boster Scientific Support

Answered: 2020-01-09

Question

Is the capture antibody for EK0749 monoclonal or polyclonal?

Verified customer

Asked: 2020-01-07

Answer

The capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Monoclonal.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the host of the capture antibody for EK0749?

Verified customer

Asked: 2020-01-07

Answer

The host of the capture antibody of the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Mouse.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the species of the immunogen capture antibody for EK0749, Human or something else?

Verified customer

Asked: 2020-01-07

Answer

The species for the immunogen capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Human.

Boster Scientific Support

Answered: 2020-01-09

Question

Is the detection antibody for EK0749 monoclonal or polyclonal?

Verified customer

Asked: 2020-01-07

Answer

The detection antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Monoclonal.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the host of the detection antibody for EK0749?

Verified customer

Asked: 2020-01-07

Answer

The host of the detection antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Mouse.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the immunogen or immunogen range (partial or full-length) of the detection antibody for EK0749?

Verified customer

Asked: 2020-01-07

Answer

The immunogen or immunogen range of the capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is G17-K17 (Accession num. P07585-4).

Boster Scientific Support

Answered: 2020-01-09

Question

What is the species of the immunogen detection antibody for EK0749? Human or something else?

Verified customer

Asked: 2020-01-07

Answer

The species of the immunogen detection antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Human.

Boster Scientific Support

Answered: 2020-01-09

Question

What is the standard for EK0749? A recombinant protein or something else? If recombinant protein, what is the host?

Verified customer

Asked: 2020-01-07

Answer

For the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749), the standard is a recombinant protein and the host is Human.

Boster Scientific Support

Answered: 2020-01-09

Question

Is the recombinant protein standard for EK0749 full length or partial?

Verified customer

Asked: 2020-01-07

Answer

The recombinant protein standard for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Full G17-K359

Boster Scientific Support

Answered: 2020-01-09

Question

What is the species of the standard for EK0749? Human or something else?

Verified customer

Asked: 2020-01-07

Answer

The species of the standard for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is Human.

Boster Scientific Support

Answered: 2020-01-09

Question

What sample type was tested with EK0749 (Serum, Plasma, or something else?) and what were the reference values?

Verified customer

Asked: 2020-01-07

Answer

The tested sample types for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) are Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, EDTA). Anticoagulant(s): heparin or EDTA.

Boster Scientific Support

Answered: 2020-01-09

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

K. Jackson

Verified customer

Asked: 2019-07-20

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-20

Question

One of our customers is interested in purchasing the kit EK0749, qty 5. Would you mind confirming the lead time and pricing? Is 1 lot of qty 5 available?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

We do not have 5 kits of the same lot available. It takes around 3 business days for manufacturing it after we receive the order.

Boster Scientific Support

Answered: 2019-06-21

Question

What is the immunogen or immunogen range (partial or full-length) of the capture antibody for EK0749?

Verified customer

Asked: 2019-01-07

Answer

The immunogen or immunogen range of the capture antibody for the Human Decorin / DCN ELISA Kit PicoKine™ (EK0749) is G17-K359 (Accession num. P07585).

Boster Scientific Support

Answered: 2019-01-09

Question

Q: if the enzyme conjugated DCN antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DCN antigen?

Y. Evans

Verified customer

Asked: 2018-09-05

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-09-05

Question

Q: is it okay to use citrate plasma as samples in Human DCN Picokine® ELISA Kit (Catalog # EK0749)?

S. Huang

Verified customer

Asked: 2017-04-29

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of DCN causing degradation of its protein structure. DCN may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the DCN ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2017-04-29

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

K. Huang

Verified customer

Asked: 2016-01-18

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-01-18

$420

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0749
$420.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.