Human Endoglycan/PODXL2 ELISA Kit PicoKine™

Human Endoglycan/PODXL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PODXL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1928
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human Endoglycan/PODXL2 ELISA Kit PicoKine™

See all Endoglycan/PODXL2 products

SKU/Catalog Number

EK1928

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Endoglycan/PODXL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human PODXL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Endoglycan/PODXL2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1928)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: G33-T500

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1928 is reactive to PODXL2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1928 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1928-CAP Anti-Human PODXL2 Pre-coated 96-well strip microplate 1
EK1928-ST Human PODXL2 Standard 2 vials, 10 ng/tube
EK1928-DA Human PODXL2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0790.1370.1910.3280.5670.9731.6122.313

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Endoglycan/PODXL2, Dilution ratio of 1:1, concentration in serum is around 1400pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Endoglycan/PODXL2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2051739579521018935983
Standard deviation8.82109.56283.969.24119.26442.74
CV (%)4.3%6.3%4.9%4.4%6.3%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Endoglycan/PODXL2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120523721021121512.475.8%
Sample 21739195018931973188891.234.8%
Sample 357956024532555195665265.844.6%
*number of samples for each test n=16.

Gene/Protein Information For PODXL2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NZ53

Gene ID

50512

Gene Name

PODXL2

Full Name

Podocalyxin-like protein 2

Weight

65.076kDa

Superfamily

podocalyxin family

Alternative Names

Endoglycan; PODLX2; podocalyxin-like 2; podocalyxin-like protein 2; PODXL2 PODXL2 EG, PODLX2 podocalyxin like 2 podocalyxin-like protein 2|endoglycan

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PODXL2, check out the PODXL2 Infographic

PODXL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PODXL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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4 Customer Q&As for Human Endoglycan/PODXL2 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

R. Mitchell

Verified customer

Asked: 2020-05-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-28

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

G. Davis

Verified customer

Asked: 2019-04-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-04-03

Question

Q: What is the optimal O.D. value for Endoglycan ELISA kit? I performed your Endoglycan ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Endoglycan even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-01-24

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Endoglycan ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Endoglycan that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2019-01-24

Question

Q: how to proceed with the analysis of ELISA data? I measured Endoglycan level in plasma.

G. Parker

Verified customer

Asked: 2017-02-17

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-02-17

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