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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.|
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Human Endostatin ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Endostatin ELISA Kit PicoKine™ (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Endostatin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0886)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: H1154-S1335
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0886 is reactive to COL18A1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
EK0886 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Endostatin
Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
|EK0886-CAP||Anti-Human COL18A1 Pre-coated 96-well strip microplate||1|
|EK0886-ST||Human COL18A1 Standard||2 vials, 20 ng/tube|
|EK0886-DA||Human COL18A1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Endostatin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Endostatin, Dilution ratio of 1:20, concentration in serum is 30-50ng/ml.
Some articles we found to cite concentrations of Endostatin in samples: 25408220, 25380724, 25279166, 21211193 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Endostatin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Endostatin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For COL18A1 (Source: Uniprot.Org, NCBI)
Collagen alpha-1(XVIII) chain
multiplexin collagen family
antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP COL18A1 GLCC, KNO, KNO1, KS collagen type XVIII alpha 1 chain collagen alpha-1(XVIII) chain|antiangiogenic agent|collagen, type XVIII, alpha 1|endostatin|multi-functional protein MFP*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on COL18A1, check out the COL18A1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for COL18A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0886 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Y, Qu Zh, Cui M, Guo C, Zhang Xm, Ma Ch, Sun Ws. Cancer Biol Ther. 2009 Mar;8(5):466-73. Epub 2009 Mar 21. Combined Endostatin And Trail Gene Transfer Suppresses Human Hepatocellular Carcinoma Growth And Angiogenesis In Nude Mice.
Fu Y, Zhao Z, Wu Y, Wu K, Xu X, Liu Y, Tong C. J Ethnopharmacol. 2012 Jan 6;139(1):26-33. Doi: 10.1016/J.Jep.2011.08.077. Epub 2011 Nov 10. Therapeutic Mechanisms Of Tongmai Dasheng Tablet On Tripterygium Glycosides Induced Rat Model For Premature...
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