Human Endostatin ELISA Kit PicoKine™

Human Endostatin ELISA Kit PicoKine™ (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0886
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Product Name

Human Endostatin ELISA Kit PicoKine™

See all Endostatin products

SKU/Catalog Number

EK0886

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Endostatin ELISA Kit PicoKine™ (96 Tests). Quantitate Human COL18A1 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Endostatin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0886)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H1154-S1335

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0886 is reactive to COL18A1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Application Guarantee

EK0886 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0886-CAP Anti-Human COL18A1 Pre-coated 96-well strip microplate 1
EK0886-ST Human COL18A1 Standard 2 vials, 20 ng/tube
EK0886-DA Human COL18A1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0680.2940.5960.9801.3231.5551.6951.916

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Endostatin, Dilution ratio of 1:20, concentration in serum is 30-50ng/ml.

Some articles we found to cite concentrations of Endostatin in samples: 25408220, 25380724, 25279166, 21211193 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Endostatin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2471523514425114625243
Standard deviation14.07109.65216.0414.3130.11319.82
CV (%)5.7%7.2%4.2%5.7%8.9%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Endostatin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 124723823220423016.066.9%
Sample 21523130913621479141886.256%
Sample 351445141585157785478336.996.1%
*number of samples for each test n=16.

Gene/Protein Information For COL18A1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P39060

Gene ID

80781

Gene Name

COL18A1

Full Name

Collagen alpha-1(XVIII) chain

Weight

178.188kDa

Superfamily

multiplexin collagen family

Alternative Names

antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP COL18A1 GLCC, KNO, KNO1, KS collagen type XVIII alpha 1 chain collagen alpha-1(XVIII) chain|antiangiogenic agent|collagen, type XVIII, alpha 1|endostatin|multi-functional protein MFP

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on COL18A1, check out the COL18A1 Infographic

COL18A1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for COL18A1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0886 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang Y, Qu Zh, Cui M, Guo C, Zhang Xm, Ma Ch, Sun Ws. Cancer Biol Ther. 2009 Mar;8(5):466-73. Epub 2009 Mar 21. Combined Endostatin And Trail Gene Transfer Suppresses Human Hepatocellular Carcinoma Growth And Angiogenesis In Nude Mice.

Fu Y, Zhao Z, Wu Y, Wu K, Xu X, Liu Y, Tong C. J Ethnopharmacol. 2012 Jan 6;139(1):26-33. Doi: 10.1016/J.Jep.2011.08.077. Epub 2011 Nov 10. Therapeutic Mechanisms Of Tongmai Dasheng Tablet On Tripterygium Glycosides Induced Rat Model For Premature...

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5 Customer Q&As for Human Endostatin ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for Endostatin ELISA kit? I used your Endostatin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Endostatin even though the O.D. values are not very high?

L. Robinson

Verified customer

Asked: 2020-09-08

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Endostatin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Endostatin that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-09-08

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-06-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-06-19

Question

Q: Can Endostatin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

C. Banerjee

Verified customer

Asked: 2019-04-20

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-04-20

Question

Q: how do I analyze ELISA data? I have obtained Endostatin level in serum.

M. Patel

Verified customer

Asked: 2019-03-04

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-03-04

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Endostatin using the EK0886 Human Endostatin Picokine® ELISA Kit?

S. Collins

Verified customer

Asked: 2018-11-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-11-11

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