Human Estrodial (E2) ELISA Kit (Competitive ELISA)

17 beta-HSD1/HSD17B1 ELISA kit

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Estrodial (E2). 96wells/kit, with removable strips.

Product Info Summary

SKU: EK7049
Size: 96wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum

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Product Name

Human Estrodial (E2) ELISA Kit (Competitive ELISA)

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SKU/Catalog Number

EK7049

Size

96wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Estrodial (E2). 96wells/kit, with removable strips.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Estrodial (E2) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7049)

Clonality of Antibodies

See Datasheet for details

Sensitivity

40 pg/ml

Assay Range

40-1400 pg/ml

Cross Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7049 is reactive to HSD17B1 in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7049 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Pre-coated 96-well strip microplate18 strips of 12 wellsGoat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate
Human Estrodial (E2) Standards (S0~S5)60.5mlEstrodial (E2) (0, 40, 120, 350, 700, 1400 pg/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300
HRP Conjugated antigen16mlHRP Conjugated Human Estrodial (E2), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300
Antibody16mlRabbit anti- Human Estrodial (E2) Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue
Controls20.5mlEstrodial (E2), 100% natural protein, 0.1% Proclin-300
20X Wash Buffer Concentrate115ml0.2M PBS containing 0.5% tween 20
Color Developing Reagent A17ml11m mol/L Urea hydrogen peroxide
Color Developing Reagent B17ml2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution17ml2mol/L Sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Data Example Images

Gene/Protein Information For HSD17B1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14061

Gene ID

3292

Gene Name

HSD17B1

Full Name

Estradiol 17-beta-dehydrogenase 1

Weight

34.95kDa

Superfamily

short-chain dehydrogenases/reductases (SDR) family

Alternative Names

17 betaHSD1; 17 beta-HSD1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E17KSR; E2DH; EC 1.1.1.62; EDH17B1; EDH17B2; EDH17B2EDHB17; EDHB17; estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; HSD17; HSD17B1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; hydroxysteroid (17-beta) dehydrogenase 1; MGC138140; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; short chain dehydrogenase/reductase family 28CE, member 1 HSD17B1 17-beta-HSD, 20-alpha-HSD, E2DH, EDH17B2, EDHB17, HSD17, SDR28C1 hydroxysteroid 17-beta dehydrogenase 1 17-beta-hydroxysteroid dehydrogenase type 1|17-beta-HSD 1|20 alpha-hydroxysteroid dehydrogenase|estradiol 17-beta-dehydrogenase 1|placental 17-beta-hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 28C member 1|short chain dehydrogenase/reductase family 28CE, member 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on HSD17B1, check out the HSD17B1 Infographic

HSD17B1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for HSD17B1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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