Product Info Summary
SKU: | EK7049 |
---|---|
Size: | 96 wells/kit |
Reactive Species: | Human |
Application: | ELISA |
Sample Types: | Serum |
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Product info
Product Name
Human Estrodial (E2) ELISA Kit (Competitive ELISA)
See all 17 beta-HSD1/HSD17B1 products
SKU/Catalog Number
EK7049
Size
96 wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Estrodial (E2). 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Estrodial (E2) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7049)
Clonality of Antibodies
See Datasheet for details
Sensitivity
40 pg/ml
Assay Range
50-1200 pg/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7049 is reactive to HSD17B1 in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7049 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Description | Quantity | Volume | Buffers |
Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Goat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate |
Human Estrodial (E2) Standards (S0~S5) | 6 | 0.5ml | Estrodial (E2) (0, 50, 120, 250, 500, 1200 pg/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300 |
HRP Conjugated antigen | 1 | 6ml | HRP Conjugated Human Estrodial (E2), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300 |
Antibody | 1 | 6ml | Rabbit anti- Human Estrodial (E2) Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue |
Controls | 2 | 0.5ml | Estrodial (E2), 100% natural protein, 0.1% Proclin-300 Range of Control 1: 84-156 pg/mL Range of Control 2: 350-650 pg/mL |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Estrodial (E2) ELISA Kit standard curve
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Protein Target Info & Infographic
Gene/Protein Information For HSD17B1 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B1
Full Name
Estradiol 17-beta-dehydrogenase 1
Weight
34.95kDa
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
17 betaHSD1; 17 beta-HSD1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E17KSR; E2DH; EC 1.1.1.62; EDH17B1; EDH17B2; EDH17B2EDHB17; EDHB17; estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; HSD17; HSD17B1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; hydroxysteroid (17-beta) dehydrogenase 1; MGC138140; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; short chain dehydrogenase/reductase family 28CE, member 1 HSD17B1 17-beta-HSD, 20-alpha-HSD, E2DH, EDH17B2, EDHB17, HSD17, SDR28C1 hydroxysteroid 17-beta dehydrogenase 1 17-beta-hydroxysteroid dehydrogenase type 1|17-beta-HSD 1|20 alpha-hydroxysteroid dehydrogenase|estradiol 17-beta-dehydrogenase 1|placental 17-beta-hydroxysteroid dehydrogenase|short chain dehydrogenase/reductase family 28C member 1|short chain dehydrogenase/reductase family 28CE, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B1, check out the HSD17B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For Human Estrodial (E2) ELISA Kit (Competitive ELISA) (EK7049)
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