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Product Info Summary
| SKU: | EK7015 |
|---|---|
| Size: | 96wells/kit |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Serum and plasma |
Product info
Product Name
Human Total Estrogens ELISA Kit
SKU/Catalog Number
EK7015
Size
96wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Total Estrogens. 96wells/kit, with removable strips.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Total Estrogens ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7015)
Clonality of Antibodies
See Datasheet for details
Assay Range
10-1500 pg/ml
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7015 is reactive to in Human samples
Validated Sample Types
Serum and plasma
Application Guarantee
EK7015 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Microwells coated with Goat Anti Rabbit IgG | 12x8x1 Microwells |
| Total Estrogens Standards | 7 vials ( ready to use) 0.250 ml |
| Anti Total Estrogens Ab Reagent | 1 vial ( ready to use) 7 ml |
| Estrogens-HRP Conjugate (20x) | 1 Vial 0.7 ml |
| Assay Diluent | 1 bottle (ready to use) 12ml |
| TMB Substrate | 1 bottle (ready to use) 12ml |
| Stop Solution | 1 bottle (ready to use) 12ml |
| 20X Wash concentrate | 1 bottle 25ml |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Data Example Images
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Human Total Estrogens ELISA Kit
Question
How much G force or RPM is needed to centrifuge the serum sample and for how long?
Verified customer
Asked: 2019-09-13
Answer
For the Human Total Estrogens ELISA Kit (EK7015), you can prepare the serum by centrifugation at 1000-2000 xg for 10 minutes.
Boster Scientific Support
Answered: 2019-09-13
Question
How long will the sample be stable at -70'C temperature storage?
Verified customer
Asked: 2019-09-13
Answer
For the Human Total Estrogens ELISA Kit (EK7015), Estrogen samples are generally stable for long period of time (6 months) frozen under -20°C but you can put it under -70°C for longer periods.
Boster Scientific Support
Answered: 2019-09-15
Question
Do you have any suggestions for an alternative if nitrogen gas is inaccessibe to evaporate the samples after extraction using EK7015? If they evaporate in a fume hood, will the samples still be okay?
Verified customer
Asked: 2019-03-06
Answer
For the process of evaporating the sample solvent using the Human Total Estrogens ELISA Kit (EK7015), it may take a longer to do it, but using indirect and mild source of heat (37oC in a sand bath) may work and we are sure your samples will be just fine but we are not sure whether it can eliminate all the solvent because at the end you will need a source of air to dry them out completely. This is just a suggestion for an alternative since you have no access to nitrogen gas.
Boster Scientific Support
Answered: 2019-03-06
