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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCGR2B/C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1312)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A46-P217
46.9 pg/ml - 3,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1312 is reactive to FCGR2B/C in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1312 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCGR2B/C
CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.
|EK1312-CAP||Anti-Human FCGR2B/C Pre-coated 96-well strip microplate||1|
|EK1312-ST||Human FCGR2B/C Standard||2 vials, 10 ng/tube|
|EK1312-DA||Human FCGR2B/C Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CD32/FCGR2b/c PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCGR2B/C, Dilution ratio of 1:1, concentration in serum is 562-1200pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FCGR2B/C in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FCGR2B/C (Source: Uniprot.Org, NCBI)
Low affinity immunoglobulin gamma Fc region receptor II-c;IgG Fc receptor II-c;CDw32;Fc-gamma RII-c;Fc-gamma-RIIc;FcRII-c;CD32;FCGR2C;CD32, FCG2, IGFR2;FCGR2B*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FCGR2B/C, check out the FCGR2B/C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCGR2B/C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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