Human Ferritin OneStep ELISA Kit

Fth1 ELISA kit

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ferritin (Ferr). 96 wells/kit.

Product Info Summary

SKU: EK7055
Size: 96wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum

Product Name

Human Ferritin OneStep ELISA Kit

See all Fth1 products

SKU/Catalog Number

EK7055

Size

96wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ferritin (Ferr). 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Ferritin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7055)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2.5 ng/mL

Assay Range

5-160 ng/mL

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7055 is reactive to Fth1 in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7055 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Anti-Human Ferritin (Ferr) Pre-coated 96-well strip microplate18 strips of 12 wellsAnti- Ferritin (Ferr) monoclonal antibody, Polystyrene micro-well plate
Human Ferritin (Ferr) Standards (S0~S5)60.5mlFerritin (Ferr) (0, 5, 15, 35, 80, 160 ng/mL), 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300
HRP Conjugated anti-Human Ferritin (Ferr) antibody16mlHRP Conjugated anti-Human Ferritin (Ferr) antibody, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300, 0.01% azophloxine
Controls20.5mlFerritin (Ferr), 100% natural protein, 0.1% Proclin-300
Color Developing Reagent A17ml11m mol/L Urea hydrogen peroxide
Color Developing Reagent B17ml2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution17ml2mol/L Sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Data Example Images

Gene/Protein Information For Fth1 (Source: Uniprot.org, NCBI)

Uniprot ID

P09528

Gene ID

14319

Gene Name

Fth1

Full Name

Ferritin heavy chain

Weight

21.067kDa

Superfamily

ferritin family

Alternative Names

apoferritin; Cell proliferation-inducing gene 15 protein; Ferritin H subunit; ferritin heavy chain; ferritin, heavy polypeptide 1; FHC; FTHEC 1.16.3.1; FTHL6MGC104426; PIG15; placenta immunoregulatory factor; PLIF; proliferation-inducing protein 15 Fth1|FHC, Fth, H-fer, HFt, MFH|ferritin heavy polypeptide 1|ferritin heavy chain|H-ferritin|ferritin H subunit|ferritin heavy chain 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Fth1, check out the Fth1 Infographic

Fth1 infographic

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In this infographic, you will see the following information for Fth1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected]

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