Human Fetuin A/AHSG PicoKine® Quick ELISA Kit

Product Name

Human Fetuin A/AHSG PicoKine® Quick ELISA Kit

See all AHSG products

SKU/Catalog Number

FEK0757

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Fetuin A/AHSG PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Human IL-8/Interleukin-8/CXCL8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Cite This Product

Human Fetuin A/AHSG PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0757)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: CHO, Immunogen sequence: Asp32-Glu143

Sensitivity

<10 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

See datasheet of FEK0757 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0757 is reactive to AHSG in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK0757 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of AHSG

Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southern blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Kit Components

Catalog number	Description	Quantity	Volume
FEK0757-CAP	Anti-tag Pre-coated 96-well Strip Microplate	1	12 strips of 8 wells
FEK0578-ST	Human AHSG Standard	2	50 ng/tube
FEK0757-A	Human AHSG Antibody A	1	3ml
FEK0757-B	Human AHSG Antibody B	1	3ml
AR1106-1	Sample Diluent	1	15ml
AR1106-7	TBS-T Wash Buffer (25x)	1	12ml
AR1104	Color Developing Reagent (TMB)	1	10ml
AR1105	Stop Solution	1	10ml
PLA-SEA	Adhesive Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional)
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.
• Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.010	0.033	0.059	0.123	0.245	0.518	1.036	1.897

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Fetuin A/AHSG PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Gene/Protein Information For AHSG (Source: Uniprot.Org, NCBI)

Alternative Names

A2HS; AHS; AHSG; alpha 2 HS glycoprotein; Alpha 2 Z globulin; Ba alpha 2 glycoprotein; FETUA; Fetuin A; Fetuin-A; HSGA

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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