Human FGF1/Fgf Acidic ELISA Kit PicoKine®

FGF acidic/FGF1 ELISA kit for Human

Human FGF1/Fgf Acidic ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0339
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human FGF1/Fgf Acidic ELISA Kit PicoKine®

View all FGF acidic/FGF1 ELISA kits

SKU/Catalog Number

EK0339

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FGF1/Fgf Acidic ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human FGF1/Fgf Acidic ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0339)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-D155

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0339 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0339 is reactive to FGF1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0339 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of FGF acidic/FGF1

Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.

Kit Components

Catalog Number Description Quantity
EK0339-CAP Anti-Human FGF1 Pre-coated 96-well strip microplate 1
EK0339-ST Human FGF1 Standard 2 vials, 10 ng/tube
EK0339-DA Human FGF1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0580.1080.1330.2130.3430.5720.9771.751

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FGF acidic/FGF1, Dilution ratio of 1:1, concentration in serum and plasma is 200-400 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FGF1/Fgf Acidic ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)59282984582701073
Standard deviation3.315.4276.753.8218.998.71
CV (%)5.4%5.4%7.8%6.6%7%9.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FGF acidic/FGF1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 159565757571.081.8%
Sample 22822752682602718.163%
Sample 398490191895794032.513.4%
*number of samples for each test n=16.

Gene/Protein Information For FGF1 (Source: Uniprot.Org, NCBI)

Gene Name

FGF1

Full Name

Fibroblast growth factor 1

Weight

17.46kDa

Superfamily

heparin-binding growth factors family

Alternative Names

AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1 FGF1 AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1 fibroblast growth factor 1 fibroblast growth factor 1|beta-endothelial cell growth factor|endothelial cell growth factor, alpha|endothelial cell growth factor, beta|fibroblast growth factor 1 (acidic)|heparin-binding growth factor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF1, check out the FGF1 Infographic

FGF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0339 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Autologous transplantation of EPCs encoding FGF1 gene promotes neovascularization in a porcine model of chronic myocardial ischemia

CRISPR/Cas9-mediated knockout of the DCL2 and DCL4 genes in Nicotiana benthamiana and its productivity of recombinant proteins

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11 Customer Q&As for Human FGF1/Fgf Acidic ELISA Kit PicoKine®

Question

Does EK0339 contain native human biological materials?

Verified customer

Asked: 2021-11-10

Answer

The Human FGF1/Fgf Acidic ELISA Kit PicoKine® (EK0339) doesn't contain native human biological materials.

Boster Scientific Support

Answered: 2021-11-11

Question

Q: What is the optimal O.D. value for FGF acidic ELISA kit? I performed your FGF acidic ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FGF acidic even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-06-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FGF acidic ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of FGF acidic that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-06-03

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

L. Banerjee

Verified customer

Asked: 2020-05-09

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-09

Question

Q: if the enzyme conjugated FGF acidic antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FGF acidic antigen?

P. Thomas

Verified customer

Asked: 2019-09-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-09-11

Question

Will EK0339 work on FGF in Bone Marrow?

Verified customer

Asked: 2019-04-19

Answer

For the Human FGF1/Fgf Acidic ELISA Kit PicoKine™ (EK0339), we haven't worked on bone marrow and they suggest the customer run a pilot test if he would like to have a try. In theory, if the expression level of the target protein in the bone marrow falls in the assay range of the kits, the kits are able to detect the protein in the sample.

Boster Scientific Support

Answered: 2019-04-22

Question

Q: can I use citrate plasma as samples in Human FGF acidic Picokine® ELISA Kit (Catalog # EK0339)?

Verified Customer

Verified customer

Asked: 2018-05-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of FGF acidic causing disruption of its protein structure. FGF acidic may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FGF acidic ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-05-13

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Moore

Verified customer

Asked: 2018-02-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-02-28

Question

Q: how do I analyze ELISA data? I measured FGF acidic level in cell lysates.

Verified Customer

Verified customer

Asked: 2018-01-27

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-27

Question

Q: can you recommend the dilution ratio of serum samples for detection of FGF acidic in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Y. Lopez

Verified customer

Asked: 2018-01-22

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FGF acidic ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-01-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2017-09-09

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-09-09

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis FGF acidic using the EK0339 Human FGF acidic Picokine® ELISA Kit?

W. Robinson

Verified customer

Asked: 2015-05-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2015-05-11

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