Human FGF21 ELISA Kit PicoKine™

Human FGF21 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0994
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human FGF21 ELISA Kit PicoKine™

See all FGF-21 products

SKU/Catalog Number

EK0994

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FGF21 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF21 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FGF21 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0994)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H29-S209

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0994 is reactive to FGF21 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0994 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0994-CAP Anti-Human FGF21 Pre-coated 96-well strip microplate 1
EK0994-ST Human FGF21 Standard 2 vials, 10 ng/tube
EK0994-DA Human FGF21 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0320.0750.1440.2040.3490.6881.1511.881

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FGF-21, Dilution ratio of 1:1, concentration in serum is around 120pg/ml.

Some articles we found to cite concentrations of FGF-21 in samples: 24813368, 24260557, 24612017 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FGF21 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5320386348217789
Standard deviation3.2312.1856.953.413.6767.06
CV (%)6.1%6%6.6%7.1%6.3%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FGF-21 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 153504955512.384.6%
Sample 220319121322020610.915.2%
Sample 386399391995593247.95.1%
*number of samples for each test n=16.

Gene/Protein Information For FGF21 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NSA1

Gene ID

26291

Gene Name

FGF21

Full Name

Fibroblast growth factor 21

Weight

22.3kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF21; FGF-21; fibroblast growth factor 21 FGF21 fibroblast growth factor 21 fibroblast growth factor 21

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FGF21, check out the FGF21 Infographic

FGF21 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FGF21: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0994 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Association of serum betatrophin with fibroblast growth factor-21 in women with polycystic ovary syndrome.

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12 Customer Q&As for Human FGF21 ELISA Kit PicoKine™

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-05-11

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-11

Question

I only have 20 µL of plasma sample. Can I dilute the sample to 100 µL for this assay?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

No if the sample is human plasma. Note that the dilution for mouse sample should be fine because its FGF21 level is higher than that in human plasma.

Boster Scientific Support

Answered: 2019-08-26

Question

Can EK0994 for measure total FGF21?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

EK0994 is able to measure total FGF21.

Boster Scientific Support

Answered: 2019-06-26

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-06-21

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification FGF-21 using the EK0994 Human FGF-21 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-04-11

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-06-20

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-06-20

Question

Q: can you recommend the dilution ratio of serum samples for detection of FGF-21 in Human serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-10-11

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FGF-21 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-10-11

Question

Q: Can FGF-21 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-03-24

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-03-24

Question

Q: how can I thaw whole blood sample for FGF-21 ELISA after freezing?

K. Gonzalez

Verified customer

Asked: 2016-10-22

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FGF-21 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-10-22

Question

Q: if the enzyme conjugated FGF-21 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FGF-21 antigen?

Verified Customer

Verified customer

Asked: 2016-04-28

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-04-28

Question

Q: how to analyze ELISA data? I have obtained FGF-21 level in serum.

Verified Customer

Verified customer

Asked: 2016-04-22

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-04-22

Question

Q: is it okay to use citrate plasma as samples in Human FGF-21 Picokine® ELISA Kit (Catalog # EK0994)?

Verified Customer

Verified customer

Asked: 2014-01-08

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of FGF-21 causing degradation of its protein structure. FGF-21 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the FGF-21 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2014-01-08

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