Human Ficolin-2 ELISA Kit PicoKine™

Human Ficolin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCN2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Product Info Summary

SKU: EK1258
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human Ficolin-2 ELISA Kit PicoKine™

See all Ficolin-2 products

SKU/Catalog Number

EK1258

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Ficolin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCN2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Ficolin-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1258)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L26-A313

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1258 is reactive to FCN2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1258 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1258-CAP Anti-Human FCN2 Pre-coated 96-well strip microplate 1
EK1258-ST Human FCN2 Standard 2 vials, 50 ng/tube
EK1258-DA Human FCN2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0080.1010.2020.3200.7681.1161.5262.050

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Ficolin-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)977681222201979652820934
Standard deviation44.94497.271354.2652.86509.181611.91
CV (%)4.6%7.3%6.1%5.4%7.8%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ficolin-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1977105811101008103850.54.8%
Sample 268126337651571626706312.974.6%
Sample 32220122603224462170422238340.221.5%
*number of samples for each test n=16.

Gene/Protein Information For FCN2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15485

Gene ID

2220

Gene Name

FCN2

Full Name

Ficolin-2

Weight

34.001kDa

Superfamily

ficolin lectin family

Alternative Names

37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin); FCN2; FCNL; FCNLficolin B; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin2; Ficolin-2; ficolin-B; ficolin-beta; Hucolin; L-Ficolin; P35; Serum lectin p35 FCN2 EBP-37, FCNL, P35, ficolin-2 ficolin 2 ficolin-2|37 kDa elastin-binding protein|L-ficolin|collagen/fibrinogen domain-containing protein 2|ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)|ficolin B|ficolin-beta|serum lectin p35

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FCN2, check out the FCN2 Infographic

FCN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Human Ficolin-2 ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-09-17

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-09-17

Question

Q: What is the optimal O.D. value for Ficolin-2 ELISA kit? I used your Ficolin-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Ficolin-2 even though the O.D. values are not very high?

E. Chakrabarti

Verified customer

Asked: 2020-09-08

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Ficolin-2 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of Ficolin-2 that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2020-09-08

Question

Q: Can Ficolin-2 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-12-08

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-12-08

Question

Q: if the enzyme conjugated Ficolin-2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Ficolin-2 antigen?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-02

Question

Q: how to thaw whole blood sample for Ficolin-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-05-21

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Ficolin-2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-05-21

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Ficolin-2 using the EK1258 Human Ficolin-2 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-06-16

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2017-06-16

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