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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human Ficolin-2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Ficolin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCN2 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Ficolin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1258)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L26-A313
0.78 ng/ml - 50 ng/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1258 is reactive to FCN2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK1258 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ficolin-2
Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.
|EK1258-CAP||Anti-Human FCN2 Pre-coated 96-well strip microplate||1|
|EK1258-ST||Human FCN2 Standard||2 vials, 50 ng/tube|
|EK1258-DA||Human FCN2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Ficolin-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Ficolin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Ficolin-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FCN2 (Source: Uniprot.Org, NCBI)
ficolin lectin family
37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin); FCN2; FCNL; FCNLficolin B; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin2; Ficolin-2; ficolin-B; ficolin-beta; Hucolin; L-Ficolin; P35; Serum lectin p35 FCN2 EBP-37, FCNL, P35, ficolin-2 ficolin 2 ficolin-2|37 kDa elastin-binding protein|L-ficolin|collagen/fibrinogen domain-containing protein 2|ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)|ficolin B|ficolin-beta|serum lectin p35*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FCN2, check out the FCN2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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