Human Ficolin-3 ELISA Kit PicoKine™

Human Ficolin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCN3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Product Info Summary

SKU: EK1259
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human Ficolin-3 ELISA Kit PicoKine™

See all FCN3 products

SKU/Catalog Number

EK1259

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Ficolin-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCN3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Ficolin-3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1259)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-R299

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1259 is reactive to FCN3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1259 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1259-CAP Anti-Human FCN3 Pre-coated 96-well strip microplate 1
EK1259-ST Human FCN3 Standard 2 vials, 50 ng/tube
EK1259-DA Human FCN3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0370.1120.2160.3700.6031.0761.3621.842

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FCN3, Dilution ratio of 1:1 1:5 , concentration in serum is around 7ng/ml around 15ng/ml.

Some articles we found to cite concentrations of FCN3 in samples: 22206485, 19208603 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Ficolin-3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12165741264801219625325739
Standard deviation80.25396.121403.4480.45450.211853.2
CV (%)6.6%6.9%5.3%6.6%7.2%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FCN3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11216134712671181125262.414.9%
Sample 257415533587664845908354.015.9%
Sample 326480265662652922150254311894.677.4%
*number of samples for each test n=16.

Gene/Protein Information For FCN3 (Source: Uniprot.Org, NCBI)

Uniprot ID

O75636

Gene ID

8547

Gene Name

FCN3

Full Name

Ficolin-3

Weight

32.903kDa

Superfamily

ficolin lectin family

Alternative Names

FCN3; FCNH; Ficolin3; Ficolin-3; HAKA1; H-Ficolin FCN3 FCNH, HAKA1 ficolin 3 ficolin-3|H-ficolin|collagen/fibrinogen domain-containing lectin 3 p35|collagen/fibrinogen domain-containing protein 3|ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FCN3, check out the FCN3 Infographic

FCN3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FCN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1259

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human Ficolin-3 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human Ficolin-3 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

9 Customer Q&As for Human Ficolin-3 ELISA Kit PicoKine™

Question

What is the immunogen sequence of EK1259?

Verified customer

Asked: 2021-01-12

Answer

The immunogen sequence of the Human FCN3 Standard in EK1259 is Q24-R299.

Boster Scientific Support

Answered: 2021-01-12

Question

Is the standard of EK1259 full-length Ficolin-3, fragment or a peptide?

Verified customer

Asked: 2021-01-12

Answer

For the Human Ficolin-3 ELISA Kit PicoKine™ (EK1259), the standard is full length Ficolin-3.

Boster Scientific Support

Answered: 2021-01-12

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-09-07

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-09-07

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-02-23

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-02-23

Question

Q: if the enzyme conjugated FCN3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FCN3 antigen?

Y. Lewis

Verified customer

Asked: 2020-02-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-02-22

Question

What is the recommended dilution ratio for human plasma samples with EK1259?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

The suggested dilution ratio is 1:100-1:1000 for human plasma samples.

Boster Scientific Support

Answered: 2020-01-03

Question

Q: can you recommend the dilution ratio of serum samples for detection of FCN3 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-11-18

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FCN3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-11-18

Question

Q: can I use citrate plasma as samples in Human FCN3 Picokine® ELISA Kit (Catalog # EK1259)?

V. Robinson

Verified customer

Asked: 2018-03-09

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FCN3 causing disruption of its protein structure. FCN3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FCN3 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-03-09

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

R. Zhao

Verified customer

Asked: 2015-01-12

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-01-12

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK1259
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.