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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human FLT4/VEGFR3 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FLT4/VEGFR3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FLT4 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FLT4/VEGFR3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0545)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y25-I776
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0545 is reactive to FLT4 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK0545 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of VEGFR3/Flt-4
Fms-related tyrosine kinase 4, also known as FLT4, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA.
|EK0545-CAP||Anti-Human FLT4 Pre-coated 96-well strip microplate||1|
|EK0545-ST||Human FLT4 Standard||2 vials, 10 ng/tube|
|EK0545-DA||Human FLT4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FLT4/VEGFR3 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FLT4/VEGFR3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of VEGFR3/Flt-4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FLT4 (Source: Uniprot.Org, NCBI)
Vascular endothelial growth factor receptor 3
protein kinase superfamily
EC 2.7.10; EC 18.104.22.168; FLT4; Flt-4; fms-related tyrosine kinase 4; LMPH1A; PCLFLT41; soluble VEGFR3 variant 1; soluble VEGFR3 variant 2; soluble VEGFR3 variant 3; Tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF R3; VEGFR3; VEGFR-3; VEGFR3Fms-like tyrosine kinase 4 FLT4 CHTD7, FLT-41, LMPH1A, LMPHM1, PCL, VEGFR-3, VEGFR3, FLT4 fms related receptor tyrosine kinase 4 vascular endothelial growth factor receptor 3|Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4|VEGF receptor-3|fms related tyrosine kinase 4|fms-like tyrosine kinase 4|primary congenital lymphedema|tyrosine-protein kinase receptor FLT4*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FLT4, check out the FLT4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FLT4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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