Human FOLR1/Alpha Fr ELISA Kit PicoKine™

Human FOLR1/Alpha Fr ELISA Kit PicoKine™ (96 Tests). Quantitate Human FOLR1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1242
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Product Name

Human FOLR1/Alpha Fr ELISA Kit PicoKine™

See all FOLR1 products

SKU/Catalog Number

EK1242

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FOLR1/Alpha Fr ELISA Kit PicoKine™ (96 Tests). Quantitate Human FOLR1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human FOLR1/Alpha Fr ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1242)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R25-S234

Sensitivity

<10 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1242 is reactive to FOLR1 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Application Guarantee

EK1242 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1242-CAP Anti-Human FOLR1 Pre-coated 96-well strip microplate 1
EK1242-ST Human FOLR1 Standard 2 vials, 10 ng/tube
EK1242-DA Human FOLR1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.99418837575015003000
O.D.0.0420.0720.1350.2220.3590.6741.2271.978

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FOLR1/Alpha Fr ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10333313061083121388
Standard deviation7.4122.3188.88.5324.96119.36
CV (%)7.2%6.7%6.8%7.9%8%8.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FOLR1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1103108100991023.53.4%
Sample 233335935533934610.83.1%
Sample 31306138614111506140271.355%
*number of samples for each test n=16.

Gene/Protein Information For FOLR1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P15328

Gene ID

2348

Gene Name

FOLR1

Full Name

Folate receptor alpha

Weight

29.819kDa

Superfamily

folate receptor family

Alternative Names

Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18 FOLR1 FBP, FOLR, FRalpha, NCFTD folate receptor alpha folate receptor alpha|FR-alpha|KB cells FBP|adult folate-binding protein|folate binding protein|folate receptor 1 (adult)|folate receptor, adult|ovarian tumor-associated antigen MOv18

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOLR1, check out the FOLR1 Infographic

FOLR1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOLR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human FOLR1/Alpha Fr ELISA Kit PicoKine™

Question

Q: is it okay to use citrate plasma as samples in Human FOLR1 Picokine® ELISA Kit (Catalog # EK1242)?

G. Bhasin

Verified customer

Asked: 2020-01-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of FOLR1 causing degradation of its protein structure. FOLR1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FOLR1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-01-12

Question

Q: how can I thaw whole blood sample for FOLR1 ELISA after freezing?

T. Young

Verified customer

Asked: 2019-08-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FOLR1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-08-01

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of FOLR1 in Human urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

V. Parker

Verified customer

Asked: 2018-12-16

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FOLR1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-12-16

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-11-01

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-11-01

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-10-25

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-10-25

Question

Q: if the enzyme conjugated FOLR1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FOLR1 antigen?

Verified Customer

Verified customer

Asked: 2016-04-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-04-01

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

P. Parker

Verified customer

Asked: 2016-01-01

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-01-01

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