Human FOLR3 ELISA Kit PicoKine®

FOLR3 ELISA kit for Human

Human FOLR3 Kit PicoKine™ (96 Tests). Quantitate Human FOLR3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EK2202
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human FOLR3 ELISA Kit PicoKine®

View all FOLR3 ELISA kits

SKU/Catalog Number

EK2202

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FOLR3 Kit PicoKine™ (96 Tests). Quantitate Human FOLR3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human FOLR3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2202)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q23-S245

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2202 is reactive to FOLR3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2202 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of FOLR3

Folate receptor gamma is a protein that in humans is encoded by the FOLR3 gene. This gene encodes a member of the folate receptor (FOLR) family of proteins, which have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Expression of this gene may be elevated in ovarian and primary peritoneal carcinoma. This gene is present in a gene cluster on chromosome 11. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK2202-CAP Anti-Human FOLR3 Pre-coated 96-well strip microplate 1
EK2202-ST Human FOLR3 Standard 2 vials, 10 ng/tube
EK2202-DA Human FOLR3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)031.262.51252505001,0002,000
O.D.0.0510.1690.2640.3970.6641.1171.8632.035

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FOLR3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)4311518052107205
Standard deviation2.245.187.561.924.612.51
CV(%)5.2%4.5%4.2%3.7%4.3%6.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FOLR3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 143485254492.865.8%
Sample 2115121108981114.974.5%
Sample 318018619920319211.716.1%
*number of samples for each test n=16.

Gene/Protein Information For FOLR3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P41439

Gene ID

2352

Gene Name

FOLR3

Full Name

Folate receptor gamma

Weight

27.885kDa

Superfamily

folate receptor family

Alternative Names

folate receptor 3 (gamma); Folate receptor 3; folate receptor gamma; FOLR3; FR-G; FR-gamma; gamma-hFR FOLR3 FR-G, FR-gamma, FRgamma, gamma-hFR folate receptor gamma folate receptor gamma|folate receptor 3 (gamma)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOLR3, check out the FOLR3 Infographic

FOLR3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOLR3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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