Human Free Triiodothyronine (FT3) ELISA Kit (Competitive ELISA)

Product Name

Human Free Triiodothyronine (FT3) ELISA Kit (Competitive ELISA)

SKU/Catalog Number

EK7053

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Competitive High Sensitivity ELISA kit for Quantitative Detection of Human FT3. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Free Triiodothyronine (FT3) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7053)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2.0 pmol/L

Assay Range

2-32 pmol/L

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7053 is reactive to in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7053 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Rabbit Anti- FT3 Antibody, Polystyrene micro-well plate
Human FT3 Standards (S1~S5)	5	1ml	FT3 (2, 4, 8, 16, 32 pmol/L), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300
Avidin-HRP conjugate	1	6ml	Avidin-HRP conjugate, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1% Proclin-300
Biotinylated antigen	1	6ml	Biotinylated FT3 analogue, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300
Controls	2	1ml	FT3, 100% natural protein, 0.1% Proclin-300
20X Wash Buffer Concentrate	1	15ml	0.2M PBS containing 0.5% tween 20
Color Developing Reagent A	1	7ml	11m mol/L Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution	1	7ml	2mol/L Sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Data Example Images

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