Human FUCA1 ELISA Kit PicoKine®

Tissue alpha-L-Fucosidase/FUCA1 ELISA kit for Human

Human FUCA1 Kit PicoKine™ (96 Tests). Quantitate Human FUCA1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml.

Product Info Summary

SKU: EK2203
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

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Product Name

Human FUCA1 ELISA Kit PicoKine®

View all Tissue alpha-L-Fucosidase/FUCA1 ELISA kits

SKU/Catalog Number

EK2203

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human FUCA1 Kit PicoKine™ (96 Tests). Quantitate Human FUCA1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human FUCA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2203)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q32-K466

Sensitivity

<1 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2203 is reactive to FUCA1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2203 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Tissue alpha-L-Fucosidase/FUCA1

Tissue alpha-L-fucosidase is an enzyme that in humans is encoded by the FUCA1 gene. The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.

Kit Components

Catalog Number Description Quantity
EK2203-CAP Anti-Human FUCA1 Pre-coated 96-well strip microplate 1
EK2203-ST Human FUCA1 Standard 2 vials, 10 ng/tube
EK2203-DA Human FUCA1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)01563126251,2502,5005,00010,000
O.D.0.0510.1020.1860.4020.8501.2401.6632.045

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human FUCA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2434869371635221079
Standard deviation10.9425.2759.976.3624.5366.90
CV(%)4.5%5.2%6.4%3.9%4.7%6.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tissue alpha-L-Fucosidase/FUCA1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12432582622872639.453.6%
Sample 248650349951250025.505.1%
Sample 393796811261051102144.94.4%
*number of samples for each test n=16.

Gene/Protein Information For FUCA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04066

Gene ID

2517

Gene Name

FUCA1

Full Name

Tissue alpha-L-fucosidase

Weight

53.689kDa

Superfamily

glycosyl hydrolase 29 family

Alternative Names

Alpha-L-fucosidase 1; Alpha-L-fucosidase I; Alpha-L-fucoside fucohydrolase 1; EC 3.2.1; EC 3.2.1.51; FUCA; FUCA1; fucosidase, alpha-L- 1, tissue; Tissue alphaLFucosidase; Tissue alpha-L-Fucosidase FUCA1 FUCA alpha-L-fucosidase 1 tissue alpha-L-fucosidase|I+/--L-fucosidase 1|a-L-fucosidase 1|alpha-L-fucosidase I|alpha-L-fucoside fucohydrolase 1|fucosidase, alpha-L- 1, tissue|tissue fucosidase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FUCA1, check out the FUCA1 Infographic

FUCA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FUCA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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