Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Product Name

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all CSF3 products

SKU/Catalog Number

EZ0360

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

CSF3 (G-CSF) is a hematopoietic cytokine that regulates granulocyte production, differentiation, and function (canonical). Assay context: EZ-Set “DIY antibody pairs” (capture/detection antibodies plus standards) used to build a sandwich ELISA for Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate); assay-build workflows can be discussed in the context of pairing/optimization strategies similar to antibody pairs development (method-context), and CSF3 inflammatory hematopoiesis readouts are often interpreted alongside upstream cytokine drivers such as IL1B (putative).

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0360)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T31-P204

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0360 is reactive to CSF3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EZ0360 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0360-CA	Mouse anti- human CSF3 monoclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0360-DA	Biotinylated goat anti- human CSF3 polyclonal antibody (Detection Antibody)	500 µl, 5.1 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0360-ST	Lyophilized recombinant human CSF3 standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.001	0.052	0.105	0.187	0.435	0.746	1.475	1.817

Data Example Images

Gene/Protein Information For CSF3 (Source: Uniprot.Org, NCBI)

Alternative Names

Granulocyte colony-stimulating factor;G-CSF;Pluripoietin;Filgrastim;Lenograstim;CSF3;C17orf33, GCSF;

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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