Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4 pg/ml.

Product Info Summary

SKU: EZ0360
Size: For 5 plates, 96 wells each, reagents only, plates are not included
Reactive Species: Human
Application: ELISA

Product Name

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all G-CSF products

SKU/Catalog Number

EZ0360

Size

For 5 plates, 96 wells each, reagents only, plates are not included

*Question: How many samples can I assay/run in this kit?

Description

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0360)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T31-P204

Sensitivity

<4 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0360 is reactive to CSF3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EZ0360 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0360-CA Mouse anti- human CSF3 monoclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0360-DA Biotinylated goat anti- human CSF3 polyclonal antibody (Detection Antibody) 500 µl, 5.1 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0360-ST Lyophilized recombinant human CSF3 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0010.0520.1050.1870.4350.7461.4751.817

Data Example Images

Gene/Protein Information For CSF3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P09919

Gene ID

1440

Gene Name

CSF3

Full Name

Granulocyte colony-stimulating factor

Weight

22.293kDa

Superfamily

IL-6 superfamily

Alternative Names

C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin CSF3 C17orf33OS, GCSF, CSF3 colony stimulating factor 3 granulocyte colony-stimulating factor|filgrastim|granulocyte-colony stimulating factor|lenograstim|pluripoietin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CSF3, check out the CSF3 Infographic

CSF3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CSF3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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