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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Human G-CSF ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human G-CSF ELISA Kit PicoKine™ (96 Tests). Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human G-CSF ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0360)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: T31-P204
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0360 is reactive to CSF3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0360 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of G-CSF
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.
|EK0360-CAP||Anti-Human CSF3 Pre-coated 96-well strip microplate||1|
|EK0360-ST||Human CSF3 Standard||2 vials, 10 ng/tube|
|EK0360-DA||Human CSF3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human G-CSF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human G-CSF ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of G-CSF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CSF3 (Source: Uniprot.Org, NCBI)
Granulocyte colony-stimulating factor
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin CSF3 C17orf33OS, GCSF, CSF3 colony stimulating factor 3 granulocyte colony-stimulating factor|filgrastim|granulocyte-colony stimulating factor|lenograstim|pluripoietin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CSF3, check out the CSF3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CSF3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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