Human Galectin-7 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-7. 96wells/kit, with removable strips.
Human Galectin-7 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human Galectin-7 ELISA Kit PicoKine™
See all Galectin-7 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Galectin-7/LGALS7 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Galectin-7/LGALS7 tissue specificity: Mainly expressed in stratified squamous epithelium.|
|Cite This Product||Human Galectin-7 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1286)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: S2-F136|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For LGALS7 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3963|
|Species Of This Entry||Human|
|Alternative Names||Galectin-7|GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1|
|Post Tranlational Modifications||Biotinylation; Cleavage; Methylation|
|Subcellular Localization||Cytoplasm. Nucleus. Secreted. May be secreted by a non-classical secretory pathway.|
|Gene Location||19q13.2, on Chromasome 19, gene sequence: NC_000019.10 (38770968..38773517, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For LGALS7 (Source: Uniprot.org, NCBI)
|Protein Function||Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.|
|Tissue Specificity||Mainly expressed in stratified squamous epithelium.|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Malignant Squamous Cell Neoplasm; Neoplasm Metastasis; Lymphoma; Tissue Adhesions; Malignant Paraganglionic Neoplasm; Tumor Progression; Cell Invasion; Corneal Diseases; Malignant Neoplasm Of Breast; Carcinogenesis; Thyroid Neoplasm; Head And Neck Neoplasms; Cell Transformation, Neoplastic; Hypopharyngeal Neoplasms; Dysplasia|
|Related Pathways||Localization; Cell Migration; Cell Growth; Cell Adhesion; Cell Proliferation; Cell Cycle; Wound Healing; Secretion; Epithelial Cell Migration; Pathogenesis; Transport; Keratinization; Cell Activation; Dna Hypomethylation; Cytokine Secretion; Immune Response; Keratinocyte Development; Keratinocyte Differentiation; Nuclear Export; Embryo Development|
|Background||Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.|
|Scientific References||PMID: 7534301 by Madsen P., et al. Cloning, expression, and chromosome mapping of human galectin-7.|
PMID: 7729568 by Magnaldo T., et al. Galectin-7, a human 14-kDa S-lectin, specifically expressed in keratinocytes and sensitive to retinoic acid.
Kit Components And Assay QC Data Details Of Human Galectin-7 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1286-CAP||96-well plate precoated with anti-Human Galectin-7 antibody||1|
|EK1286-ST||lyophilized recombinant Human Galectin-7 standard||10ng/tube|
|EK1286-DA||biotinylated anti-Human Galectin-7 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1286 Human Galectin-7 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human Galectin-7 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human Galectin-7 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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