Human GAS6 ELISA Kit PicoKine®

GAS6 ELISA kit for Human

Human GAS6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human GAS6 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1992
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin or EDTA)

Product Name

Human GAS6 ELISA Kit PicoKine®

View all GAS6 ELISA kits

SKU/Catalog Number

EK1992

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GAS6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human GAS6 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GAS6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1992)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A49-A678

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1992 is reactive to GAS6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin or EDTA)

Application Guarantee

EK1992 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GAS6

Growth arrest-specific 6, also known as GAS6, is a human gene coding for the Gas6 protein. It is mapped to 13q34. This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. Gas6 is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation.

Kit Components

Catalog Number Description Quantity
EK1992-CAP Anti-Human GAS6 Pre-coated 96-well strip microplate 1
EK1992-ST Human GAS6 Standard 2 vials, 10 ng/tube
EK1992-DA Human GAS6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0340.1260.1770.2920.5100.9341.5362.280

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect GAS6, Dilution ratio of 1:10, concentration in serum is around 8ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GAS6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9943414031194821746
Standard deviation4.2628.2165.947.3833.26123.97
CV (%)4.3%6.5%4.7%6.2%6.9%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GAS6 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 19992101100983.533.6%
Sample 243442441145943217.594.0%
Sample 31403140715241413143650.493.5%
*number of samples for each test n=16.

Gene/Protein Information For GAS6 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14393

Gene ID

2621

Gene Name

GAS6

Full Name

Growth arrest-specific protein 6

Weight

74.925kDa

Alternative Names

AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6 GAS6 AXLLG, AXSF growth arrest specific 6 growth arrest-specific protein 6|AXL receptor tyrosine kinase ligand|AXL stimulatory factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GAS6, check out the GAS6 Infographic

GAS6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GAS6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human GAS6 ELISA Kit PicoKine®

Question

Q: if the enzyme conjugated GAS6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GAS6 antigen?

Verified Customer

Verified customer

Asked: 2020-09-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-09-14

Question

Q: is it okay to use citrate plasma as samples in Human GAS6 Picokine® ELISA Kit (Catalog # EK1992)?

C. Edwards

Verified customer

Asked: 2020-04-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of GAS6 causing degradation of its protein structure. GAS6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the GAS6 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-04-22

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of GAS6 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

B. Hall

Verified customer

Asked: 2019-05-27

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the GAS6 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-05-27

Question

Q: how to thaw whole blood sample for GAS6 ELISA after freezing?

S. Campbell

Verified customer

Asked: 2016-12-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GAS6 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-12-17

Question

Q: What is the optimal O.D. value for GAS6 ELISA kit? I used your GAS6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain GAS6 even though the O.D. values are not very high?

D. Jha

Verified customer

Asked: 2016-08-30

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GAS6 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of GAS6 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2016-08-30

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