Human GCNT1 ELISA Kit PicoKine®

GCNT1 ELISA kit for Human

Human GCNT1 Kit PicoKine™ (96 Tests). Quantitate Human GCNT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2204
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human GCNT1 ELISA Kit PicoKine®

View all GCNT1 ELISA kits

SKU/Catalog Number

EK2204

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GCNT1 Kit PicoKine™ (96 Tests). Quantitate Human GCNT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GCNT1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2204)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R33-H428

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2204 is reactive to GCNT1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2204 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of GCNT1

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the GCNT1 gene. This gene is a member of the beta-1,6-N-acetylglucosaminyltransferase gene family. It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene coding this enzyme was originally mapped to 9q21, but was later localized to 9q13. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Kit Components

Catalog Number Description Quantity
EK2204-CAP Anti-Human GCNT1 Pre-coated 96-well strip microplate 1
EK2204-ST Human GCNT1 Standard 2 vials, 10 ng/tube
EK2204-DA Human GCNT1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)062.51252505001,0002,0004,000
O.D.0.0590.0820.1090.1880.4110.6351.3682.048

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GCNT1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)109204389116214472
Standard deviation5.0110.6113.237.4212.2022.66
CV (%)4.6%5.2%3.4%6.4%5.7%4.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of GCNT1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11091051031121074.614.3%
Sample 220422224123622611.515.1%
Sample 338934241544439822.265.6%
*number of samples for each test n=16.

Gene/Protein Information For GCNT1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q02742

Gene ID

2650

Gene Name

GCNT1

Full Name

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Weight

49.799kDa

Superfamily

glycosyltransferase 14 family

Alternative Names

beta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N-acetylglucosaminyltransferase; beta-1,6-N-acetylglucosaminyltransferase; C2GNT1; C2GNTbeta-1,3-galactosyl-O-glycosyl-glycoproteinbeta-1,6-N--acetylglucosaminyltransferase; C2GNT-L; core 2 beta1,6 N-acetylglucosaminyltransferase-I; core 2 beta-1,6-N-acetylglucosaminyltransferase I; core 2 branching enzyme; Core 2 GNT; Core 2-branching enzyme; core2-GlcNAc-transferase; EC 2.4.1.102; G6NT; GCNT1; glucosaminyl (N-acetyl) transferase 1, core 2; glucosaminyl (N-acetyl) transferase 1, core 2(beta-1,6-N-acetylglucosaminyltransferase); MGC126335; MG GCNT1 C2GNT, C2GNT-L, C2GNT1, G6NT, NACGT2, NAGCT2 glucosaminyl (N-acetyl) transferase 1 beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase|beta-1,6-N-acetylglucosaminyltransferase|core 2 GnT|core 2 beta-1,6-N-acetylglucosaminyltransferase I|core 2 beta1,6 N-acetylglucosaminyltransferase-I|core 2 branching enzyme|core2-GlcNAc-transferase|glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GCNT1, check out the GCNT1 Infographic

GCNT1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GCNT1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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