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Product Info Summary
| SKU: | EK2075 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human GFRA3 ELISA Kit PicoKine®
View all GFR alpha-3/GDNF R alpha-3 ELISA kits
SKU/Catalog Number
EK2075
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GFRA3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human GFRA3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GFRA3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2075)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D32-N374
Sensitivity
<20 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2075 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2075 is reactive to GFRA3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2075 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GFR alpha-3/GDNF R alpha-3
GDNF family receptor alpha-3 (GFRα3), also known as the artemin receptor, is a protein that in humans is encoded by the GFRA3 gene. It is mapped to 5q31.2. The protein encoded by this gene is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2075-CAP | Anti-Human GFRA3 Pre-coated 96-well strip microplate | 1 |
| EK2075-ST | Human GFRA3 Standard | 2 vials, 10 ng/tube |
| EK2075-DA | Human GFRA3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.036 | 0.075 | 0.144 | 0.245 | 0.468 | 0.821 | 1.315 | 1.908 |
Data Example Images
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Human GFRA3 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GFR alpha-3/GDNF R alpha-3, Dilution ratio of 1:1, concentration in serum and plasma is around 80 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GFRA3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 49 | 225 | 1375 | 62 | 303 | 1421 |
| Standard deviation | 2.50 | 11.93 | 75.63 | 4.09 | 20.60 | 102.31 |
| CV (%) | 5.1% | 5.3% | 5.5% | 6.6% | 6.8% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GFR alpha-3/GDNF R alpha-3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 49 | 53 | 50 | 54 | 51 | 2.06 | 4.0% |
| Sample 2 | 225 | 222 | 233 | 208 | 222 | 9.02 | 4.0% |
| Sample 3 | 1375 | 1355 | 1340 | 1235 | 1326 | 54.12 | 4.0% |
Protein Target Info & Infographic
Gene/Protein Information For GFRA3 (Source: Uniprot.Org, NCBI)
Gene Name
GFRA3
Full Name
GDNF family receptor alpha-3
Weight
44.511kDa
Superfamily
GDNFR family
Alternative Names
GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF R alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR alpha3; GFR alpha-3; GFRA3; GFRa-3; GFR-alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3; GPI-linked receptor GFRA3 GDNFR3 GDNF family receptor alpha 3 GDNF family receptor alpha-3|GDNF receptor alpha-3|GDNFR-alpha-3|GFR-alpha-3|GPI-linked receptor|glial cell line-derived neurotrophic factor receptor alpha-3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GFRA3, check out the GFRA3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GFRA3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GFRA3 ELISA Kit PicoKine® (EK2075)
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No publications found for EK2075
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Human GFRA3 ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for GFR alpha-3 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-11-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GFR alpha-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-11-28
Question
Q: if the enzyme conjugated GFR alpha-3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GFR alpha-3 antigen?
H. Johnson
Verified customer
Asked: 2020-10-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-10-04
Question
Q: how do I analyze ELISA data? I measured GFR alpha-3 level in plasma.
S. Hill
Verified customer
Asked: 2019-08-29
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-08-29
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-07-21
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-21
Question
Q: can you suggest the dilution ratio of serum samples for detection of GFR alpha-3 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the GFR alpha-3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-03-11
Question
Q: can I use EDTA plasma as samples in Human GFR alpha-3 Picokine® ELISA Kit (Catalog # EK2075)?
Verified Customer
Verified customer
Asked: 2019-01-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of GFR alpha-3 causing degradation of its protein structure. GFR alpha-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the GFR alpha-3 ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-01-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Walker
Verified customer
Asked: 2018-08-13
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-08-13
