Human GLB1 ELISA Kit PicoKine®

beta-Galactosidase-1/GLB1 ELISA kit for Human

Human GLB1 Kit PicoKine™ (96 Tests). Quantitate Human GLB1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EK2208
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA)

Product Name

Human GLB1 ELISA Kit PicoKine®

View all beta-Galactosidase-1/GLB1 ELISA kits

SKU/Catalog Number

EK2208

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GLB1 Kit PicoKine™ (96 Tests). Quantitate Human GLB1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GLB1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2208)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-V677

Sensitivity

<10 pg/ml

Assay Range

93.7 pg/ml - 6,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2208 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2208 is reactive to GLB1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA)

Application Guarantee

EK2208 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of beta-Galactosidase-1/GLB1

Galactosidase, beta 1, also known as GLB1, is a protein which in humans is encoded by the GLB1 gene. This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome.

Kit Components

Catalog Number Description Quantity
EK2208-CAP Anti-Human GLB1 Pre-coated 96-well strip microplate 1
EK2208-ST Human GLB1 Standard 2 vials, 10 ng/tube
EK2208-DA Human GLB1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration(pg/ml)093.7187.53757501,5003,0006,000
O.D.0.0410.0740.1530.3050.6120.9861.4252.054

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GLB1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)14186239641387563785
Standard deviation9.0237.93273.52844.6196.82
CV(%)6.4%4.4%6.9%5.8%5.9%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of beta-Galactosidase-1/GLB1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11411381551461458.275.7%
Sample 286286688082285845.455.3%
Sample 339643564384139523830222.155.8%
*number of samples for each test n=16.

Gene/Protein Information For GLB1 (Source: Uniprot.Org, NCBI)

Gene Name

GLB1

Full Name

Beta-galactosidase

Weight

76.075kDa

Superfamily

glycosyl hydrolase 35 family

Alternative Names

Acid beta-galactosidase; beta-galactosidase; betaGalactosidase1; beta-Galactosidase-1; EBP; EC 3.2.1.23; elastin receptor 1 (67kD); Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase, beta 1; GLB1; Lactase; MPS4B GLB1 EBP, ELNR1, MPS4B galactosidase beta 1 beta-galactosidase|acid beta-galactosidase|elastin binding protein|elastin receptor 1, 67kDa|lactase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GLB1, check out the GLB1 Infographic

GLB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GLB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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