Human GM-CSF High Sensitivity ELISA Kit PicoKine®

Product Name

Human GM-CSF High Sensitivity ELISA Kit PicoKine®

SKU/Catalog Number

HSEK0364

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human GM-CSF ELISA Kit PicoKine® (96 Tests). Quantitate Human CSF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human GM-CSF High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # HSEK0364)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: A18-E144

Capture/Detection Antibodies

polyclonal antibody from goat|polyclonal antibody from goat

Sensitivity

<0.1 pg/ml

Assay Range

1.56-100pg/ml

Standard Dilution Instructions

See datasheet of HSEK0364 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

HSEK0364 is reactive to CSF2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

HSEK0364 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CSF2

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts ly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Kit Components

Catalog Number	Description	Quantity
HSEK0364-CAP	Anti-Human CSF2 Pre-coated 96-well strip microplate	1
HSEK0364-ST	Human CSF2 Standard	2 vials, 10 ng/tube
HSEK0364-DA	Human CSF2 Biotinylated antibody (100x)	100ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100ul
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.034	0.111	0.171	0.298	0.52	0.999	1.69	2.736

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human GM-CSF High Sensitivity ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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