Human Gp130/IL6ST ELISA Kit PicoKine™

Human Gp130/IL6ST ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6ST in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0367
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human Gp130/IL6ST ELISA Kit PicoKine™

See all gp130/CD130 products

SKU/Catalog Number

EK0367

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Gp130/IL6ST ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL6ST in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Gp130/IL6ST ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0367)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L24-E619

Sensitivity

<10 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0367 is reactive to IL6ST in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0367 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0367-CAP Anti-Human IL6ST Pre-coated 96-well strip microplate 1
EK0367-ST Human IL6ST Standard 2 vials, 10 ng/tube
EK0367-DA Human IL6ST Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01252505001000200040008000
O.D.0.0330.0990.1490.2460.4310.7351.2601.910

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Gp130/IL6ST ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1861115338417810763150
Standard deviation13.273.59156.6%612.6380.7198.45
CV (%)7.1%6.6%4.6%7.1%7.5%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of gp130/CD130 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11862031982071987.883.9%
Sample 21115115010731056109836.673.3%
Sample 33384350735373330343985.342.4%
*number of samples for each test n=16.

Gene/Protein Information For IL6ST (Source: Uniprot.Org, NCBI)

Uniprot ID

P40189

Gene ID

3572

Gene Name

IL6ST

Full Name

Interleukin-6 receptor subunit beta

Weight

103.537kDa

Superfamily

type I cytokine receptor family

Alternative Names

CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha IL6ST CD130, CDW130, GP130, HIES4, IL-6RB, sGP130 interleukin 6 cytokine family signal transducer interleukin-6 receptor subunit beta|CD130 antigen|IL-6 receptor subunit beta|IL-6R subunit beta|gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form|gp130, oncostatin M receptor|interleukin receptor beta chain|interleukin-6 signal transducer|membrane glycoprotein 130|membrane glycoprotein gp130|oncostatin-M receptor subunit alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL6ST, check out the IL6ST Infographic

IL6ST infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL6ST: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Human Gp130/IL6ST ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for CD130 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-05-19

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD130 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-05-19

Question

Q: how do I analyze ELISA data? I have performed CD130 level in plasma.

Z. Liu

Verified customer

Asked: 2020-03-31

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-31

Question

Q: can I use heparin plasma as samples in Human CD130 Picokine® ELISA Kit (Catalog # EK0367)?

Verified Customer

Verified customer

Asked: 2018-06-25

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD130 causing disruption of its protein structure. CD130 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD130 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-06-25

Question

Q: if the enzyme conjugated CD130 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD130 antigen?

Verified Customer

Verified customer

Asked: 2018-02-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-02-09

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD130 using the EK0367 Human CD130 Picokine® ELISA Kit?

W. Maharaj

Verified customer

Asked: 2015-08-27

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2015-08-27

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2015-02-02

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-02-02

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