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Product Info Summary
| SKU: | EK0367 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human Gp130/IL6ST ELISA Kit PicoKine®
SKU/Catalog Number
EK0367
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Gp130/IL6ST ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6ST in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Gp130/IL6ST ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0367)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L24-E619
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0367 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0367 is reactive to IL6ST in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0367 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL6ST
GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membrane-bound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0367-CAP | Anti-Human IL6ST Pre-coated 96-well strip microplate | 1 |
| EK0367-ST | Human IL6ST Standard | 2 vials, 10 ng/tube |
| EK0367-DA | Human IL6ST Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.029 | 0.078 | 0.143 | 0.234 | 0.424 | 0.846 | 1.289 | 1.909 |
Data Example Images
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Human Gp130/IL6ST PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL6ST, Dilution ratio of 1:200, concentration in serum and plasma is around 190-446 ng/ml..
Some articles we found to cite concentrations of IL6ST in samples: 11778537 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Gp130/IL6ST ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 186 | 1115 | 3384 | 178 | 1076 | 3150 |
| Standard deviation | 13.2 | 73.59 | 156.6%6 | 12.63 | 80.7 | 198.45 |
| CV (%) | 7.1% | 6.6% | 4.6% | 7.1% | 7.5% | 6.3% |
Specific Publications For Human Gp130/IL6ST ELISA Kit PicoKine® (EK0367)
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Customer Reviews
0 Reviews For Human Gp130/IL6ST ELISA Kit PicoKine®
Customer Q&As
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7 Customer Q&As for Human Gp130/IL6ST ELISA Kit PicoKine®
Question
Does EK0367 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human Gp130/IL6ST ELISA Kit PicoKine® (EK0367) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: how to thaw whole blood sample for CD130 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-05-19
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD130 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-05-19
Question
Q: how do I analyze ELISA data? I have performed CD130 level in plasma.
Z. Liu
Verified customer
Asked: 2020-03-31
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-03-31
Question
Q: can I use heparin plasma as samples in Human CD130 Picokine® ELISA Kit (Catalog # EK0367)?
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD130 causing disruption of its protein structure. CD130 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD130 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-06-25
Question
Q: if the enzyme conjugated CD130 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD130 antigen?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-09
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD130 using the EK0367 Human CD130 Picokine® ELISA Kit?
W. Maharaj
Verified customer
Asked: 2015-08-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2015-08-27
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-02-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-02-02
