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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA).|
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Human GSTA ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human GSTA ELISA Kit PicoKine™ (96 Tests). Quantitate Human GSTA1 in cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GSTA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1625)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A2-F222
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1625 is reactive to GSTA1 in Human samples
Validated Sample Types
cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA).
EK1625 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GSTA1
GSTA encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.
|EK1625-CAP||Anti-Human GSTA1 Pre-coated 96-well strip microplate||1|
|EK1625-ST||Human GSTA1 Standard||2 vials, 10 ng/tube|
|EK1625-DA||Human GSTA1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human GSTA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GSTA1, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GSTA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of GSTA1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
|Sample . 2||1176||1300||1194||1293||1240||56.16||4.5%%|
Gene/Protein Information For GSTA1 (Source: Uniprot.Org, NCBI)
Glutathione S-transferase A1
EC 22.214.171.124; glutathione S-alkyltransferase A1; glutathione S-aryltransferase A1; glutathione S-transferase 2; glutathione S-transferase A1; glutathione S-transferase alpha 1; glutathione S-transferase Ha subunit 1; GST class-alpha member 1; GST HA subunit 1; GST, class alpha, 1; GST2; GSTA1-1; GST-epsilon; GTH1; MGC131939; S-(hydroxyalkyl)glutathione lyase A1 GSTA1 GST-epsilon, GST2-1, GTH1, GSTA1 glutathione S-transferase alpha 1 glutathione S-transferase A1|13-hydroperoxyoctadecadienoate peroxidase|GST HA subunit 1|GST class-alpha member 1|S-(hydroxyalkyl)glutathione lyase A1|androst-5-ene-3,17-dione isomerase|glutathione S-alkyltransferase A1|glutathione S-aryltransferase A1|glutathione S-transferase 2|glutathione S-transferase Ha subunit 1|testicular tissue protein Li 80*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on GSTA1, check out the GSTA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GSTA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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