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Product Info Summary
| SKU: | FEK0773 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0773
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human HAI-2/SPINT2 PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human SPINT2 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0773)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M1-K197
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0773 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0773 is reactive to SPINT2 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates
Application Guarantee
FEK0773 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HAI-2/SPINT2
Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0773-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0773-ST | Human HAI-2/SPINT2 Standard | 2 | 10 ng/tube |
| FEK0773-A | Human SPINT2 Antibody A | 1 | 3ml |
| FEK0773-B | Human SPINT2 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.096 | 0.157 | 0.202 | 0.298 | 0.473 | 0.787 | 1.322 | 2.105 |
Data Example Images
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Human HAI-2/SPINT2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 59 | 211 | 923 | 61 | 224 | 890 |
| Standard deviation | 3.07 | 10.97 | 61.84 | 3.54 | 12.32 | 62.3 |
| CV (%) | 5.2% | 5.2% | 6.7% | 5.8% | 5.5% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HAI-2/SPINT2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 59 | 51 | 45 | 47 | 51 | 2.22 | 4.4% |
| Sample 2 | 211 | 221 | 223 | 199 | 214 | 8.33 | 3.9% |
| Sample 3 | 923 | 961 | 826 | 917 | 907 | 53.5 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For SPINT2 (Source: Uniprot.Org, NCBI)
Gene Name
SPINT2
Full Name
Kunitz-type protease inhibitor 2
Weight
28.228kDa
Alternative Names
HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2 SPINT2 DIAR3, HAI-2, HAI2, Kop, PB serine peptidase inhibitor, Kunitz type 2 kunitz-type protease inhibitor 2|hepatocyte growth factor activator inhibitor type 2|serine protease inhibitor, Kunitz type, 2|testicular tissue protein Li 183
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SPINT2, check out the SPINT2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SPINT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human HAI-2/SPINT2 PicoKine® Quick ELISA Kit (FEK0773)
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