Overview
| Product Name |
Human ICAM-2 ELISA Kit PicoKine™
See more ICAM-2/CD102 products |
| Catalog# |
EK0999 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Human ICAM-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ICAM2 in cell culture supernatants, serum and plasma ( heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human ICAM-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0999)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum and plasma ( heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
312 pg/ml - 20,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: K25-Q223 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For ICAM2 (Source: Uniprot.org, NCBI)
| Uniprot Id | P13598 |
|---|
| NCBI Gene Id | 3384 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ICAM2 |
|---|
| Protein Name | Intercellular adhesion molecule 2 |
|---|
| Superfamily | immunoglobulin superfamily |
|---|
| Alternative Names | ICAM-2/CD102|CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2 |
|---|
| Molecular Weight | 30654 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ICAM2, check out the ICAM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ICAM2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ICAM2 infographic
Kit Components And Assay QC Data Details Of Human ICAM-2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0999-CAP |
Anti-Human ICAM2 Pre-coated 96-well strip microplate | 1 |
| EK0999-ST | Human ICAM2 Standard | 2 vials, 20 ng/tube |
| EK0999-DA | Human ICAM2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0999 Human ICAM-2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.118 | 0.232 | 0.343 | 0.508 | 0.910 | 1.463 | 1.951 | 2.583 |
We measured random samples of Human ICAM-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 640 | 2403 | 7918 | 592 | 2447 | 7410 |
| Standard deviation | 38.4 | 151.38 | 443.4 | 38.48 | 196.5% | 437.19 |
| CV (%) | 6% | 8.1%% | 5.6% | 6.5% | 8.1% | 5.9% |
We measured the performance consistency of Human ICAM-2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 640 | 642 | 715 | 684 | 670 | 31.24 | 4.6% |
| Sample 2 | 2403 | 2237 | 2185 | 2586 | 2352 | 156.89 | 6.6% |
| Sample 3 | 7918 | 7803 | 8915 | 7900 | 8134 | 453.02 | 5.5% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce... |
PubMed ID 21647942 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0999 Human ICAM-2 ELISA Kit PicoKine™
7 Related Questions
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2020-11-19
Boster Scientific Support
Answered: 2020-11-19
Question
Q: is it okay to use citrate plasma as samples in Human CD102 Picokine® ELISA Kit (Catalog # EK0999)?
Verified Customer
Asked: 2019-08-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of CD102 causing disruption of its protein structure. CD102 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD102 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: if the enzyme conjugated CD102 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD102 antigen?
Verified Customer
Asked: 2019-05-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: how to analyze ELISA data? I measured CD102 level in plasma.
Verified Customer
Asked: 2019-03-27
Boster Scientific Support
Answered: 2019-03-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD102 using the EK0999 Human CD102 Picokine® ELISA Kit?
Verified Customer
Asked: 2018-09-04
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-09-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD102 ELISA after freezing?
T. Zhou
Asked: 2018-05-06
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD102 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-05-06
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD102 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Asked: 2017-05-29
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD102 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-05-29
