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Human ICAM-2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ICAM-2. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human ICAM-2 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0999 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, serum and plasma( heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 312pg/ml-20,000pg/ml |
Introduction
NAD (P) Dependent Steroid Dehydrogenase-Like (NSDHL) takes part in synthesis of cholesterol. NSDHL is involved in one of a few steps which convert lanosterol to cholesterol by removing a carbon atom and three hydrogen atoms (a methyl group). NSDHL is also a vital element of cell membranes and myelin, the fatty covering that insulates nerve cells.
Overview
| Product Name | Human ICAM-2 ELISA Kit PicoKine™
See all ICAM-2/CD102 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0999 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Cd102/ICAM2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd102/ICAM2 tissue specificity: |
| Cite This Product | Human ICAM-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0999) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma( heparin, EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 312pg/ml-20,000pg/ml |
| Immunogen/Standard | Expression system for standard: NSO; Immunogen sequence: K25-Q223 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Gene/Protein Basic Information For ICAM2 (Source: Uniprot.org, NCBI)
| Uniprot Id | P13598 |
|---|---|
| NCBI Gene Id | 3384 |
| Species Of This Entry | Human |
| Gene Name | ICAM2 |
| Protein Name | Intercellular adhesion molecule 2 |
| Superfamily | immunoglobulin superfamily |
| Alternative Names | ICAM-2/CD102|CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2 |
| Molecular Weight | 30654 |
| Post Tranlational Modifications | Phosphorylation; Glycosylation; Cleavage; Reduction; Biotinylation; Acetylation; Oxidation |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. Cell projection, microvillus. Co-localizes with RDX, EZR and MSN in microvilli. |
| Gene Location | 17q23.3, on Chromasome 17, gene sequence: NC_000017.11 (64002595..64020634, complement) |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ICAM2 (Source: Uniprot.org, NCBI)
| Protein Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. |
|---|---|
| Related Diseases | Tissue Adhesions; Inflammation; Neoplasms; Malignant Neoplasms; Hiv Infections; Carcinoma; Immunologic Deficiency Syndromes; Lymphoma; Neoplasm Metastasis; Extravasation; Leukemia; Arthritis; Rheumatoid Arthritis; Von Willebrand Disease; Tumor Angiogenesis; Autoimmune Reaction; Inflammatory Response; Asthma; Cell Invasion; Virus Diseases |
| Related Pathways | Cell Adhesion; Cell Activation; Pathogenesis; Cell Proliferation; Immune Response; Localization; T Cell Activation; T Cell Proliferation; Cytolysis; Cytokine Production; Angiogenesis; Diapedesis; Inflammatory Response; Secretion; Locomotion; Cell Migration; Cell Differentiation; Cell-cell Adhesion; Endothelial Cell Activation; Glycosylation |
| Background | Intercellular adhesion molecule 2(ICAM2), also known as CD102(Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. |
| Scientific References | PMID: 2497351 by Staunton D.E., et al. Functional cloning of ICAM-2, a cell adhesion ligand for LFA-1 homologous to ICAM-1. PMID: 19892738 by Xu G., et al. Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini. |
Kit Components And Assay QC Data Details Of Human ICAM-2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0999-CAP | 96-well plate precoated with anti-Human ICAM-2/CD102 antibody | 1 |
| EK0999-ST | lyophilized recombinant Human ICAM-2/CD102 standard | 20ng/tube |
| EK0999-DA | biotinylated anti-Human ICAM-2/CD102 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0999 Human ICAM-2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.118 | 0.232 | 0.343 | 0.508 | 0.910 | 1.463 | 1.951 | 2.583 |
We measured random samples of Human ICAM-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 640 | 2403 | 7918 | 592 | 2447 | 7410 |
| Standard deviation | 38.4 | 151.38 | 443.4 | 38.48 | 196.5% | 437.19 |
| CV(%) | 6% | 8.1%% | 5.6% | 6.5% | 8.1% | 5.9% |
We measured the performance consistency of Human ICAM-2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 640 | 642 | 715 | 684 | 670 | 31.24 | 4.6% |
| Sample 2 | 2403 | 2237 | 2185 | 2586 | 2352 | 156.89 | 6.6% |
| Sample 3 | 7918 | 7803 | 8915 | 7900 | 8134 | 453.02 | 5.5% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all ICAM-2/CD102 primary antibodies,Other Recommended Resources
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Total number of citations: 1
| Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce... | PubMed ID 21647942 |
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Contact Information
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| (888) 466-3604 |
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