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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma ( heparin, EDTA).|
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Human ICAM-2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human ICAM-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ICAM2 in cell culture supernatants, serum and plasma ( heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human ICAM-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0999)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: K25-Q223
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0999 is reactive to ICAM2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA).
EK0999 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ICAM-2/CD102
Intercellular adhesion molecule 2 (ICAM2), also known as CD102 (Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
|EK0999-CAP||Anti-Human ICAM2 Pre-coated 96-well strip microplate||1|
|EK0999-ST||Human ICAM2 Standard||2 vials, 20 ng/tube|
|EK0999-DA||Human ICAM2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human ICAM-2 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human ICAM-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of ICAM-2/CD102 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For ICAM2 (Source: Uniprot.Org, NCBI)
Intercellular adhesion molecule 2
CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2 ICAM2 CD102 intercellular adhesion molecule 2 intercellular adhesion molecule 2|ICAM-2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ICAM2, check out the ICAM2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ICAM2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0999 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...
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