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Product Info Summary
| SKU: | EK1452 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®
View all Iduronate 2-Sulfatase/IDS ELISA kits
SKU/Catalog Number
EK1452
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine™ (96 Tests). Quantitate Human IDS in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1452)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S26-P550
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1452 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1452 is reactive to IDS in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1452 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Iduronate 2-Sulfatase/IDS
Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1452-CAP | Anti-Human IDS Pre-coated 96-well strip microplate | 1 |
| EK1452-ST | Human IDS Standard | 2 vials, 10 ng/tube |
| EK1452-DA | Human IDS Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.030 | 0.071 | 0.165 | 0.291 | 0.510 | 0.802 | 1.350 | 1.921 |
Data Example Images
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Human IDS PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Iduronate 2-Sulfatase/IDS, Dilution ratio of 1:10, concentration in serum and plasma is 13.7-38.3 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 336 | 1290 | 4175 | 344 | 1377 | 4351 |
| Standard deviation | 22.84 | 54.18 | 324.25 | 27.86 | 68.85 | 417.69 |
| CV (%) | 6.8% | 4.2% | 7.8% | 8.1% | 5% | 9.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Iduronate 2-Sulfatase/IDS in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 336 | 299 | 345 | 321 | 325 | 17.41 | 5.3% |
| Sample 2 | 1290 | 1156 | 1326 | 1367 | 1284 | 79.16 | 6.1% |
| Sample 3 | 4175 | 4541 | 4703 | 4646 | 4516 | 205.41 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For IDS (Source: Uniprot.Org, NCBI)
Gene Name
IDS
Full Name
Iduronate 2-sulfatase
Weight
61.873kDa
Superfamily
sulfatase family
Alternative Names
Alpha-L-iduronate sulfate sulfatase; EC 3.1.6.13; IDS; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate 2-sulfatase; idursulfase; MPS2; S; SIDS IDS ID2S, MPS2, SIDS iduronate 2-sulfatase iduronate 2-sulfatase|alpha-L-iduronate sulfate sulfatase|iduronate 2-sulfatase 14 kDa chain|iduronate 2-sulfatase 42 kDa chain|idursulfase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IDS, check out the IDS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IDS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine® (EK1452)
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I measured IDS level in serum.
Verified Customer
Verified customer
Asked: 2020-10-08
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-10-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. Brown
Verified customer
Asked: 2020-08-31
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-31
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
B. Zhou
Verified customer
Asked: 2020-03-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-31
Question
Q: can I use citrate plasma as samples in Human IDS Picokine® ELISA Kit (Catalog # EK1452)?
Verified Customer
Verified customer
Asked: 2019-04-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IDS causing degradation of its protein structure. IDS may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IDS ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-04-21
Question
Q: how to thaw whole blood sample for IDS ELISA after freezing?
C. Mangal
Verified customer
Asked: 2018-11-01
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IDS for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-11-01
Question
This is Mei from Immusoft. I recently purchased human IDS PicoKine Elisa kit from your company (Cat Number: EK1452). I have one question about your protocol. In your assay procedure step 3, there is no wash step (you specify "Do not wash" in the summary). I am interested in knowing what the matter of it is. Usually for other Elisa assay, we want to wash a couple of times to get rid of unbound protein. I did test your kit, and I think it works, but the variability is a little high, and the standards is a little curved (not a good linear). So I am trying to troubleshoot it. Any of your input will be highly appreciated.
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
Regarding the wash step in step 3, our lab technicains found there wasn't any significant difference in result valus between washing and without washing
Boster Scientific Support
Answered: 2018-08-09
Question
Q: if the enzyme conjugated IDS antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IDS antigen?
Z. Adams
Verified customer
Asked: 2018-05-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-05-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IDS using the EK1452 Human IDS Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-04-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-04-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-05-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-05-08
Question
Q: can you suggest the dilution ratio of serum samples for detection of IDS in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
B. Wilson
Verified customer
Asked: 2015-04-18
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IDS ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2015-04-18
