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Product Info Summary
| SKU: | EK0397 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
Product info
Product Name
Human IL-2/Interleukin-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0397
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-2/Interleukin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL2 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-2/Interleukin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0397)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A21-T153
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0397 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0397 is reactive to IL2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).
Application Guarantee
EK0397 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-2
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0397-CAP | Anti-Human IL2 Pre-coated 96-well strip microplate | 1 |
| EK0397-ST | Human IL2 Standard | 2 vials, 10 ng/tube |
| EK0397-DA | Human IL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.030 | 0.065 | 0.123 | 0.212 | 0.336 | 0.609 | 1.031 | 1.798 |
Data Example Images
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Human IL-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-2, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of IL-2 in samples: 22040441 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-2/Interleukin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 158 | 484 | 28 | 169 | 474 |
| Standard deviation | 1.27 | 8.84 | 30 | 1.14 | 10.98 | 38.39 |
| CV (%) | 4.1% | 5.6% | 6.2% | 4.1% | 6.5% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 29 | 28 | 30 | 29 | 1.11 | 3.8% |
| Sample 2 | 158 | 160 | 151 | 163 | 158 | 4.41 | 2.7% |
| Sample 3 | 484 | 469 | 488 | 480 | 480 | 7.08 | 1.4% |
Protein Target Info & Infographic
Gene/Protein Information For IL2 (Source: Uniprot.Org, NCBI)
Gene Name
IL2
Full Name
Interleukin-2
Weight
17.628kDa
Superfamily
IL-2 family
Alternative Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF IL2 IL-2, TCGF, lymphokine interleukin 2 interleukin-2|T cell growth factor|aldesleukin|involved in regulation of T-cell clonal expansion
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL2, check out the IL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-2/Interleukin-2 ELISA Kit PicoKine® (EK0397)
Hello CJ!
EK0397 has been cited in 43 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of Astragalus membranaceus roots supplementation on growth performance, serum antioxidant and immune response in finishing lambs
miR-375 inhibits IFN-γ-induced programmed death 1 ligand 1 surface expression in head and neck squamous cell carcinoma cells by blocking JAK2/STAT1 signaling
Construction of chimeric antigen receptor‑modified T cells targeting EpCAM and assessment of their anti‑tumor effect on cancer cells
Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome
IL-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line H446
Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer
Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical Cystectomy: preliminary results
The TGF‐β/miR‐31/CEACAM1‐S axis inhibits CD4+CD25+ Treg differentiation in systemic lupus erythematosus
Impact of hydrosalpinx fluid on early human embryos
IL-2 and IL-2R gene polymorphisms and immune function in people residing in areas with high background radiation, Yangjiang, China
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Customer Reviews
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1 Reviews For Human IL-2/Interleukin-2 ELISA Kit PicoKine®
0
The standards and ELISA kit worked great.--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE
Excellent
Source: Biocompare.com
| Applications | Detection of secreted IL-2 in media of Jurkat T cells |
|---|---|
| Sample | Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep) |
| Detection | -- |
"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."
Jason Somarelli
Verified customer
Submitted 2016-01-05
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
9 Customer Q&As for Human IL-2/Interleukin-2 ELISA Kit PicoKine®
Question
What would be the effects of incubating at room temperature versus 37°C for EK0397?
Verified customer
Asked: 2021-01-13
Answer
For the Human IL-2 ELISA Kit PicoKine™ (EK0397), it takes longer to incubate at room temperature than at 37°C. https://www.bosterbio.com/datasheet?sku=EK0397 Please see the following detailed information. 4. Cover with the plate sealer provided and incubate for 120 minutes at RT (or 90 min. at 37 °C) 7. Cover with plate sealer and incubate for 90 minutes at RT (or 60 minutes at 37°C). 9. Add 100 µl of the prepared 1x Avidin-Biotin-Peroxidase Complex into each well. Cover with the plate sealer provided and incubate for 40 minutes at RT (or 30 minutes at 37°C). 11. Add 90 µl of Color Developing Reagent to each well. Cover with the plate sealer provided and incubate in the dark for 30 minutes at RT (or 15-25 minutes at 37°C).
Boster Scientific Support
Answered: 2021-01-13
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-08-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-30
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IL-2 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-08-15
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-08-15
Question
Q: if the enzyme conjugated IL-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-2 antigen?
L. Clark
Verified customer
Asked: 2020-06-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-06-06
Question
We have a customer that has a question regarding your product EK0397 https://www.bosterbio.com/datasheet_new.php?sku=EK0397 The customer is asking whether the standard can be reused after he has added ddH2O to the standard?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We have got two questions for this inquiry. 1. What is the volume of the
Boster Scientific Support
Answered: 2020-03-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-03
Question
Q: is it okay to use heparin plasma as samples in Human IL-2 Picokine® ELISA Kit (Catalog # EK0397)?
Verified Customer
Verified customer
Asked: 2019-01-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-2 causing degradation of its protein structure. IL-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-2 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-01-12
Question
Q: What is the optimal O.D. value for IL-2 ELISA kit? I performed your IL-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-10-22
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-2 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-2 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2017-10-22
Question
Q: how to thaw whole blood sample for IL-2 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-06-03
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-06-03
