Human IL-20 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-20. 96wells/kit, with removable strips. Cited in 4 publication(s).
Human IL-20 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Human IL-20 ELISA Kit PicoKine™
See all IL-20 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human IL20 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL20 tissue specificity: Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only. .|
|Cite This Product||Human IL-20 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0599)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: L25-E176|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For IL20 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||50604|
|Species Of This Entry||Human|
|Alternative Names||IL-20|Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907|
|Post Tranlational Modifications||Disulfide bond|
|Gene Location||1q32.1, on Chromasome 1, gene sequence: NC_000001.11 (206865247..206869223)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL20 (Source: Uniprot.org, NCBI)
|Protein Function||Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3.|
|Tissue Specificity||Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only.|
|Related Diseases||Dysequilibrium Syndrome; Inflammatory Response; Psoriasis; Hepatitis; Inflammation; Hepatitis, Chronic; Dermatitis, Atopic; Hepatitis C, Chronic; Hepatitis B; Carcinoma; Malignant Neoplasms; Systemic Onset Juvenile Chronic Arthritis; Asthma; Airway Disease; Bronchiolitis; Pustulosis Of Palms And Soles; Arthritis, Juvenile Rheumatoid; Infective Disorder; Respiratory Syncytial Virus Bronchiolitis|
|Related Pathways||Pathogenesis; Pigmentation; Inflammatory Response; Senescence; Replicative Senescence; Cytokine Production; Cell Cycle; Cellular Senescence; Aging; Keratinocyte Differentiation; Cell Cycle Arrest|
|Background||Interleukin-20(IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.|
|Scientific References||PMID: 11163236 by Blumberg H., et al. Interleukin 20: discovery, receptor identification, and role in epidermal function.|
PMID: 16511554 by Hsieh M.Y., et al. Interleukin-20 promotes angiogenesis in a direct and indirect manner.
Kit Components And Assay QC Data Details Of Human IL-20 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0599-CAP||96-well plate precoated with anti-Human IL-20 antibody||1|
|EK0599-ST||lyophilized recombinant Human IL-20 standard||10ng/tube|
|EK0599-DA||biotinylated anti-Human IL-20 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0599 Human IL-20 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-20 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human IL-20 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 4
|Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...||PubMed ID 24593676|
|Zhou Qb, Jin Yl, Jia Q, Zhang Y, Li Ly, Liu P, Liu Yt. Inflammation. 2014 Feb;37(1):107-15. Doi: 10.1007/S10753-013-9717-9. Baicalin Attenuates Brain Edema In A Rat Model Of Intracerebral Hemorrhage.||PubMed ID 23974988|
|Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...||PubMed ID 22863994|
|Xia Hz, Du Wd, Wu Q, Chen G, Zhou Y, Tang Xf, Tang Hy, Liu Y, Yang F, Ruan J, Xu S, Zuo Xb, Zhang Xj. Mol Carcinog. 2012 Aug;51(8):597-607. Doi: 10.1002/Mc.20828. Epub 2011 Jul 20. E-Selectin Rs5361 And Fcgr2A Rs1801274 Variants Were Associated Wi...||PubMed ID 21780194|
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