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Product Info Summary
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Human Insulin OneStep ELISA Kit
*Question: How many samples can I assay/run in this kit?
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Insulin. 96 wells/kit.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Insulin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7000)
Clonality of Antibodies
See Datasheet for details
There is no detectable cross-reactivity.
EK7000 is reactive to INS in Human samples
Validated Sample Types
EK7000 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|Anti-Human Insulin Pre-coated 96-well strip microplate||1||8 strips of 12 wells||Anti-Insulin monoclonal antibody, Polystyrene micro-well plate|
|Human Insulin Standards (S1~S5)||5||-||Insulin (10,16,30,60,100mIU/L), 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300|
|HRP Conjugated anti-Human Insulin antibody||1||6ml||HRP Conjugated anti-Human Insulin antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300|
|Controls||2||-||100% natural protein, 0.1%Proclin-300|
|20X Wash Buffer Concentrate||1||15ml||0.2M PBS containing 0.5% tween 20|
|Color Developing Reagent A||1||7ml||11m mol/L Urea hydrogen peroxide|
|Color Developing Reagent B||1||7ml||2m mol/L 3,3'5,5’-Tetramethylbenzidine|
|Stop Solution||1||7ml||2mol/L Sulphuric acid|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Insulin ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect INS, Dilution ratio of 1:1, concentration in serum is around 40mIU/l.
Gene/Protein Information For INS (Source: Uniprot.Org, NCBI)
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on INS, check out the INS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for INS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK7000 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
“Oil-soluble” reversed lipid nanoparticles for oral insulin delivery
Evaluation of adherence to Mediterranean diet and association with clinical and biological markers in an Italian population
Effects of dancing compared to walking on cardiovascular risk and functional capacity of older women: A randomized controlled trial
PDX1 transfection induces human adipose derived stem cells differentiation into islet-like cells: what is the benefit for diabetic rats?
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