Human Insulin OneStep ELISA Kit

Product Name

Human Insulin OneStep ELISA Kit

See all INS products

SKU/Catalog Number

EK7000

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Insulin. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Insulin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7000)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2.0 mIU/L

Assay Range

10-160 mIU/L

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7000 is reactive to INS in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7000 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity	Volume	Buffers
Anti-Human Insulin Pre-coated 96-well strip microplate	1	8 strips of 12 wells	Anti-Insulin monoclonal antibody, Polystyrene micro-well plate
Human Insulin Standards (S0~S5)	6	0.5ml	Insulin (0, 10, 20, 40, 80, 160mIU/L), 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300
HRP Conjugated anti-Human Insulin antibody	1	6ml	HRP Conjugated anti-Human Insulin antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300
Controls	2	0.5ml	100% natural protein, 0.1%Proclin-300, Range of Control 1: 11.6-19.2mIU/L Range of Control 2: 54.4-90.6mIU/L
20X Wash Buffer Concentrate	1	15ml	0.2M PBS containing 0.5% tween 20
Color Developing Reagent A	1	7ml	11m mol/L Urea hydrogen peroxide
Color Developing Reagent B	1	7ml	2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution	1	7ml	2mol/L Sulphuric acid
Plate Sealers	2	Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (mIU/L)	0	10	20	40	80	160
O.D.	0.000	0.244	0.415	0.828	1.378	2.131

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect INS, Dilution ratio of 1:1, concentration in serum is around 40 mIU/l.

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