Human Insulin OneStep ELISA Kit

INS ELISA kit

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Insulin. 96 wells/kit. Cited in 4 publication(s).

Product Info Summary

SKU: EK7000
Size: 96 wells/kit
Reactive Species: Human
Application: ELISA
Sample Types: Serum

Product Name

Human Insulin OneStep ELISA Kit

See all INS products

SKU/Catalog Number

EK7000

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Insulin. 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Insulin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7000)

Clonality of Antibodies

See Datasheet for details

Sensitivity

2.0 mIU/L

Assay Range

10-100 mIU/L

Cross Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7000 is reactive to INS in Human samples

Validated Sample Types

Serum

Application Guarantee

EK7000 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

DescriptionQuantityVolumeBuffers
Anti-Human Insulin Pre-coated 96-well strip microplate18 strips of 12 wellsAnti-Insulin monoclonal antibody, Polystyrene micro-well plate
Human Insulin Standards (S1~S5)5-Insulin (10,16,30,60,100mIU/L), 0.02M PBS, 20% new-born calf serum, 0.1%Proclin-300
HRP Conjugated anti-Human Insulin antibody16mlHRP Conjugated anti-Human Insulin antibody, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1%Proclin-300
Controls2-100% natural protein, 0.1%Proclin-300
20X Wash Buffer Concentrate115ml0.2M PBS containing 0.5% tween 20
Color Developing Reagent A17ml11m mol/L Urea hydrogen peroxide
Color Developing Reagent B17ml2m mol/L 3,3'5,5’-Tetramethylbenzidine
Stop Solution17ml2mol/L Sulphuric acid
Plate Sealers2Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (mIU/L)10163060100
O.D.0.1160.1550.3481.0712.108

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect INS, Dilution ratio of 1:1, concentration in serum is around 40mIU/l.

Gene/Protein Information For INS (Source: Uniprot.Org, NCBI)

Uniprot ID

P01308

Gene ID

3630

Gene Name

INS

Full Name

Insulin

Weight

11.981kDa

Superfamily

insulin family

Alternative Names

IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin INS IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4 insulin insulin|preproinsulin|proinsulin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on INS, check out the INS Infographic

INS infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for INS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK7000 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

“Oil-soluble” reversed lipid nanoparticles for oral insulin delivery

Evaluation of adherence to Mediterranean diet and association with clinical and biological markers in an Italian population

Effects of dancing compared to walking on cardiovascular risk and functional capacity of older women: A randomized controlled trial

PDX1 transfection induces human adipose derived stem cells differentiation into islet-like cells: what is the benefit for diabetic rats?

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2 Customer Q&As for Human Insulin OneStep ELISA Kit

Question

Would EK7000 cross-react with beagle serum?

Verified customer

Asked: 2021-02-21

Answer

The Human Insulin OneStep ELISA Kit (EK7000) is validated with human samples only and we are uncertain if the kit has any cross-reactivity with beagle serum. We suggest to run a pilot test with the specific samples but we can't guarantee the accuracy of the result data.

Boster Scientific Support

Answered: 2021-02-22

Question

Hello Boster, I just want to inquire if you have any information about the cross-reactivity of the EK7000 Human Insulin OneStep ELISA Kit with snake plasma samples particularly the Green tree python.

Verified Customer

Verified customer

Asked: 2016-08-12

Answer

As we check about your query, we do not have any data for reactivity on Green tree python. If you want to check cross-reactivity to other species please contact our technical support for more information. But if the sequences are 90%+ identical, there is a high chance of cross-reactivity for your species of interest.

Boster Scientific Support

Answered: 2016-08-12

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