Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit

Product Name

Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit

See all PTH products

SKU/Catalog Number

EK7081

Size

96 wells/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96 wells/kit.

Storage & Handling

Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7081)

Clonality of Antibodies

See Datasheet for details

Assay Range

0 - 1000 pg/ml

Cross-Reactivity

There is no detectable cross-reactivity.

Reactive Species

EK7081 is reactive to PTH in Human samples

Validated Sample Types

Serum and Plasma

Application Guarantee

EK7081 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Description	Quantity
1. Microwells coated with Streptavidin	12x8x1
2. PTH Standard 6: 1 Vial (lyophilized)	0.75 ml
3. PTH Controls: 2 Vials (lyophilized)	0.75ml
4. Anti-PTH-Biotin Reagent: 1 Bottle (Ready to use)	7 ml
5. Anti-PTH-HRP Enzyme Conjugate: 1 Bottle (Ready to use)	7 ml
6. Assay Diluent: 1 Bottle (ready to use)	5 ml
6. TMB Substrate: 1 Bottle (Ready to use)	12 ml
7. Stop Solution: 1 Bottle (Ready to use)	12 ml
8. 20X Wash Solution: 1 Bottle	25 ml

Standard Concentrations and example data

	Conc. pg/mL	OD 450 nm
Std 1	0	0.029
Std 2	11.1	0.082
Std 3	33.3	0.178
Std 4	100	0.459
Std 5	300	1.078
Std 6	900	2.535

LIMITATIONS OF THE PROCEDURE

1. Do not use sodium azide as preservative. Sodium azide inhibits HRP enzyme activities.
2. The PTH ELISA kit has exhibited no “high dose hook effect” with high dose spiked samples. However, samples with intact PTH levels greater than the highest calibrator should be diluted and re-assayed for correct value.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

25 µl/well

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect PTH, ELISA Target remove.

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