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Product Info Summary
| SKU: | EK2227 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine®
SKU/Catalog Number
EK2227
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Intergrin Alpha 5/CD49E PicoKine ELISA Kit® (96 Tests). Quantitate Human Intergrin Alpha 5/CD49E in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2227)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: F42-Y995
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2227 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2227 is reactive to ITGA5 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2227 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ITGA5
Integrin alpha-5 is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2227-CAP | Anti-Human ITGA5 Pre-coated 96-well strip microplate | 1 |
| EK2227-ST | Human ITGA5 Standard | 2 vials, 10 ng/tube |
| EK2227-DA | Human ITGA5 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.024 | 0.041 | 0.084 | 0.237 | 0.564 | 1.033 | 1.525 | 2.022 |
Data Example Images
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Human CD49E PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 242 | 502 | 2865 | 207 | 579 | 4142 |
| Standard deviation | 18.27 | 39.11 | 115.46 | 13.50 | 32.48 | 277.93 |
| CV(%) | 7.6% | 7.8% | 4% | 6.5% | 5.6% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ITGA5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 242 | 166 | 180 | 215 | 201 | 9.23 | 4.6% |
| Sample 2 | 502 | 527 | 467 | 385 | 470 | 32.92 | 7% |
| Sample 3 | 2865 | 4075 | 4582 | 2563 | 3521 | 196.49 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For ITGA5 (Source: Uniprot.Org, NCBI)
Gene Name
ITGA5
Full Name
Integrin alpha-5
Weight
114.536kDa
Superfamily
integrin alpha chain family
Alternative Names
Integrin alpha 5 beta 1; VLA-5 ITGA5 CD49e, FNRA, VLA-5, VLA5A integrin subunit alpha 5 integrin alpha-5|CD49 antigen-like family member E|ITGA5/SLC9B1 fusion|fibronectin receptor subunit alpha|fibronectin receptor, alpha polypeptide|fibronectin receptor, alpha subunit|integrin alpha-F|integrin, alpha 5 (fibronectin receptor, alpha polypeptide)|very late activation protein 5, alpha subunit
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ITGA5, check out the ITGA5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ITGA5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Intergrin Alpha 5/CD49E ELISA Kit PicoKine® (EK2227)
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