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- Table of Contents
ITGA5 antibody (A01911) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of ITGA5 using anti-ITGA5 antibody (A01911).
ITGA5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGA5 Antibody (A01911) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
ITGA5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGA5 Antibody (A01911) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Designing a Western blot for ITGA5? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ITGA5 Western Blot GuideBundle Save
Anti-Integrin alpha 5/ITGA5 Antibody Picoband®
ITGA5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC, WB |
Tested Applications
ITGA5 antibody was positively detected by WB in:
human A549 whole cell lysates, human Hela whole cell lysates, human placenta tissue lysates, rat brain tissue lysates, rat PC-12 whole cell lysates, mouse brain tissue lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ITGA5 antibody was positively detected by IHC in:
human placenta tissue, rat bladder tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5 μg/ml
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Integrin alpha 5/ITGA5 Antibody Picoband®
View all ITGA5 Antibodies
View all ITGA5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
114.5 kDa
Background of ITGA5
Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Cite This Product
Anti-Integrin alpha 5/ITGA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01911)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
96%
High Homology
Sheep
95%
High Homology
Cow
94%
High Homology
Dog
94%
High Homology
Mouse
91%
High Homology
Rat
90%
High Homology
Zebrafish
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ITGA5 antibody
Immunohistochemistry (IHC) protocol for ITGA5 antibody
Publications
Application:
Species:
Bioinformatic and experimental analysis reveals ITGA5 as a driver of ovarian clear cell carcinoma progression and platinum resistance
Published: 2026-05-20
Journal: 3 Biotech
Application: WB,IHC
Impact Factor: 3.10
Species: Human
PMID: 42179928
Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization
Published: 2022-02-09
Journal: Microbiology Spectrum
Application: WB
Impact Factor: 9.04
Species: Chicken
PMID: 35138149
Revealing the pathogenic changes of PAH based on multiomics characteristics
Published: 2019-07-22
Journal: Journal of Translational Medicine
Application: WB
Impact Factor: 4.10
Species: Rat
PMID: 31331330
A Fibrin Matrix Promotes the Differentiation of EMSCs Isolated from Nasal Respiratory Mucosa to Myelinating Phenotypical Schwann-Like Cells
Published: 2015-02-04
Journal: MOLECULES AND CELLS
Application: WB
Impact Factor: 2.09
Species: Rat
PMID: 25666351
Calreticulin overexpression correlates with integrin-α5 and transforming growth factor-β1 expression in the atria of patients with rheumatic valvular disease and atrial fibrillation
Published: 2013-03-07
Journal: INTERNATIONAL JOURNAL OF CARDIOLOGY
Application: WB,IP
Impact Factor: 5.51
Species: Human
PMID: 23465222
Review
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Product Promise
We promise Anti-Integrin alpha 5/ITGA5 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01911
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 22, 2026