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Product Info Summary
| SKU: | A01911 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Integrin alpha 5/ITGA5 Antibody Picoband®
SKU/Catalog Number
A01911
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Integrin alpha 5/ITGA5 Antibody Picoband® catalog # A01911. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha 5/ITGA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01911)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01911 is reactive to ITGA5 in Human, Mouse, Rat
Observed Molecular Weight
130 kDa
Calculated molecular weight
114.5 kDa
Background of ITGA5
Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01911 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5 μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml
ELISA, 0.1-0.5 μg/ml
Positive Control
WB: human A549 whole cell lysates, human Hela whole cell lysates, human placenta tissue lysates, rat brain tissue lysates, rat PC-12 whole cell lysates, mouse brain tissue lysates
IHC: human placenta tissue, rat bladder tissue
Validation Images & Assay Conditions
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Western blot analysis of ITGA5 using anti-ITGA5 antibody (A01911).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA5 antigen affinity purified polyclonal antibody (Catalog # A01911) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA5 at approximately 130 kDa. The expected band size for ITGA5 is at 115 kDa.
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IHC analysis of ITGA5 using anti-ITGA5 antibody (A01911).
ITGA5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGA5 Antibody (A01911) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of ITGA5 using anti-ITGA5 antibody (A01911).
ITGA5 was detected in a paraffin-embedded section of rat bladder tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGA5 Antibody (A01911) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Integrin alpha 5/ITGA5 Antibody Picoband® (A01911)
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Customer Q&As
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8 Customer Q&As for Anti-Integrin alpha 5/ITGA5 Antibody Picoband®
Question
I am interested to purchase the Anti-Integrin alpha 5/ITGA5 Antibody Picoband to use it in cell culture to block the function of a5 integrin. What is the recommended concentration for this antibody in this kind of experiment? Does the product binds the cytoplasmic or extracellular domain?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The product Anti-Integrin alpha 5/ITGA5 Antibody Picoband was used to inhibit VLA5-mediated functions in vitro. The application varies so we recommend to determine the optimum working dilution of the product that is appropriate for a specific need. Unfortunately we cannot provide information about the epitope and were the product binds. However, we can validate that the product sourced is external and no epitope mapping was performed, so the location of binding is unknown. But we can suggest that this clone reacts with the a5 chain of the integrin a5b1 fibronectin receptor on a variety of mouse cells and cell lines
Boster Scientific Support
Answered: 2019-12-17
Question
See attached the WB image, lot number and protocol we used for liver using anti-Integrin alpha 5/ITGA5 antibody A01911. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
I see that the anti-Integrin alpha 5/ITGA5 antibody A01911 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Integrin alpha 5/ITGA5 antibody A01911. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-14
Question
Would anti-Integrin alpha 5/ITGA5 antibody A01911 work for IHC-P with liver?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
According to the expression profile of liver, ITGA5 is highly expressed in liver. So, it is likely that anti-Integrin alpha 5/ITGA5 antibody A01911 will work for IHC-P with liver.
Boster Scientific Support
Answered: 2018-05-08
Question
I am interested in to test anti-Integrin alpha 5/ITGA5 antibody A01911 on mouse liver for research purposes, then I may be interested in using anti-Integrin alpha 5/ITGA5 antibody A01911 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Moore
Verified customer
Asked: 2017-09-06
Answer
The products we sell, including anti-Integrin alpha 5/ITGA5 antibody A01911, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-06
Question
Is a blocking peptide available for product anti-Integrin alpha 5/ITGA5 antibody (A01911)?
Verified Customer
Verified customer
Asked: 2017-06-16
Answer
We do provide the blocking peptide for product anti-Integrin alpha 5/ITGA5 antibody (A01911). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-06-16
Question
I am looking for the function blocking antibody for integrin alpha 5 to use in human cells. Is your product Anti-Integrin alpha 5/ITGA5 Antibody Picoband would be suitable for it?
Verified Customer
Verified customer
Asked: 2014-08-29
Answer
Yes. We would recommend to use Anti-Integrin alpha 5/ITGA5 Antibody Picoband, since it is the only anti-integrin alpha 5 antibody listed in our catalogue whose inhibitory activity has been previously tested. Thus, it is suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T and B lymphocytes on fibronectin. For your further reference please check this on PUBMID: 16548586 ; Mardilovich A et al. Design of a novel fibronectin-mimetic peptide-amphiphile for functionalized biomaterials. Langmuir 22:3259-64 (2006)
Boster Scientific Support
Answered: 2014-08-29
