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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates and serum.|
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Human Laminin ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Laminin ELISA Kit PicoKine™ (96 Tests). Quantitate Human LAMA1; LAMB1; LAMC1 in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Laminin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0434)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: from human fibroblasts
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0434 is reactive to LAMA1; LAMB1; LAMC1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates and serum.
EK0434 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0434-CAP||Anti-Human LAMA1; LAMB1; LAMC1 Pre-coated 96-well strip microplate||1|
|EK0434-ST||Human LAMA1; LAMB1; LAMC1 Standard||2 vials, 10 ng/tube|
|EK0434-DA||Human LAMA1; LAMB1; LAMC1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human LAMA1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LAMA1, Dilution ratio of 1:10, concentration in serum is 5-9ng/ml.
Some articles we found to cite concentrations of LAMA1 in samples: 20593050 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Laminin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of LAMA1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LAMA1; LAMB1; LAMC1 (Source: Uniprot.Org, NCBI)
LAMA1; LAMB1; LAMC1
Laminin subunit alpha-1
LAMA; Laminin A chain; laminin subunit alpha-1; laminin, alpha 1; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-LAM alpha; S-laminin subunit alpha LAMA1 LAMA, PTBHS, S-LAM-alpha laminin subunit alpha 1 laminin subunit alpha-1|S-LAM alpha|S-laminin subunit alpha|laminin A chain|laminin, alpha 1|laminin-3 subunit alpha*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LAMA1; LAMB1; LAMC1, check out the LAMA1; LAMB1; LAMC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LAMA1; LAMB1; LAMC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0434 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus
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