Human Laminin ELISA Kit PicoKine®

Laminin ELISA kit for Human

Human Laminin ELISA Kit PicoKine™ (96 Tests). Quantitate Human Laminin in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml. Cited in 4 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0434
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates and serum.

Product Name

Human Laminin ELISA Kit PicoKine®

SKU/Catalog Number

EK0434

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Laminin ELISA Kit PicoKine™ (96 Tests). Quantitate Human Laminin in cell culture supernatants, cell lysates and serum. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Laminin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0434)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: from human fibroblasts

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0434 is reactive to Laminin in Human samples

Validated Sample Types

cell culture supernatants, cell lysates and serum.

Application Guarantee

EK0434 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Laminin

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Kit Components

Catalog Number Description Quantity
EK0434-CAP Anti-Human Laminin Pre-coated 96-well strip microplate 1
EK0434-ST Human Laminin Standard 2 vials, 10 ng/tube
EK0434-DA Human Laminin Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0950.1370.1680.2470.4250.6241.2842.010

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Laminin, Dilution ratio of 1:10, concentration in serum is 5-9ng/ml.

Some articles we found to cite concentrations of Laminin in samples: 20593050 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Laminin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3181051446229510154289
Standard deviation23.2147.29348.0326.2558.87411.74
CV (%)7.3%4.5%7.8%8.9%5.8%9.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Laminin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131836431437334226.477.7%
Sample 21051124211221215115775.96.5%
Sample 344624109460645444430192.374.3%
*number of samples for each test n=16.

EK0434 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus

Multicellular 3D Neurovascular Unit Model for Assessing Hypoxia and Neuroinflammation Induced Blood-Brain Barrier Dysfunction

Cytotoxic sesquiterpenoids against hepatic stellate cell line LX2 from Artemisia lavandulaefolia

High fibrosis indices in cerebrospinal fluid of patients with shunt-dependent post-traumatic chronic hydrocephalus

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1 Reviews For Human Laminin ELISA Kit PicoKine®

0

Easy to Use Human Laminin ELISA Kit--John Ong, University of Cambridge Engineering Graduate Student

Excellent

Source: Biocompare.com

Application Testing laminin concentration in hydrogel
Starting Material Hydrogel
Tips Supernatant used for ELISA

"Positive:(1) Very straight forward and easy to use kits.(2) Accurate results(3) Removable well-strips so experiments can be staggered.Negative:(1) Price point - could be cheaper(2) Reagents have to be used within 1 month upon defrosting."

Verified customer

Submitted

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13 Customer Q&As for Human Laminin ELISA Kit PicoKine®

Question

Would EK0434 work for the quantification of laminin 332 (or laminin-5) in the cell supernatant?

Verified customer

Asked: 2021-02-01

Answer

The Human Laminin ELISA Kit PicoKine™ (EK0434) detects total laminin and it is not specific to any isoform like laminin 332 or laminin-5.

Boster Scientific Support

Answered: 2021-02-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-05-09

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-09

Question

Q: which procedure should I follow in order to thaw whole blood sample for LAMA1; LAMB1; LAMC1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-05-09

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LAMA1; LAMB1; LAMC1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-05-09

Question

Q: can you recommend the dilution ratio of serum samples for detection of LAMA1; LAMB1; LAMC1 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LAMA1; LAMB1; LAMC1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-01-31

Question

Which subunits of laminin does this kit recognize? Keywords: chain, isoform, subunits

Verified Customer

Verified customer

Asked: 2019-11-25

Answer

Our Human Laminin PicoKine™ ELISA Kit EK0434 is the Laminin pan specific to all Laminin subunits. The kit can recognize alpha, beta, and gamma subunits of laminin.

Boster Scientific Support

Answered: 2019-11-25

Question

Has EK0434 been tested for condition media in samples?

Verified customer

Asked: 2019-08-19

Answer

Unfortunately, we have not tested the Human Laminin ELISA Kit PicoKine™ (EK0434) for condition media, so we cannot guarantee that would work. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-08-19

Question

Would EK0434 kit be suitable for detection of laminin 1, 2 and 8 (Either beta-1 or gamma-1) and which chain or isomer is this kit specific for?

Verified customer

Asked: 2019-08-16

Answer

The Human Laminin ELISA Kit PicoKine EK0434 is able to detect all forms of Laminin. It is not isoform specific.

Boster Scientific Support

Answered: 2019-08-16

Question

Are the capture and detection antibodies for EK0434 rabbit polyclonals? Do they have the same immunogen and are they both towards the native human protein?

Verified customer

Asked: 2019-05-03

Answer

Both capture and detection antibodies of the Human Laminin ELISA Kit PicoKine™ (EK0434) have the same immunogen. They are both towards the native human protein.

Boster Scientific Support

Answered: 2019-05-03

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring LAMA1; LAMB1; LAMC1 using the EK0434 Human LAMA1; LAMB1; LAMC1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-01-20

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-01-20

Question

My main concert is if this kit would be suitable for the laminin isomer I will be using it for. Which chain/isomer is this kit specific for?

Verified Customer

Verified customer

Asked: 2018-10-01

Answer

EK0434 is able to detect all forms of Laminin. It is not isoform specific.

Boster Scientific Support

Answered: 2018-10-01

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-02-06

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

L. Gandhi

Verified customer

Asked: 2016-08-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-08-04

Question

The protocol for EK0434 states that PBS or TBS should be used as wash buffer. Is there any difference between PBS and TBS (i.e. depending on sample type)?

Verified Customer

Verified customer

Asked: 2014-09-04

Answer

PBS and TBS are only different in recipes. You are free to choose either of them.

Boster Scientific Support

Answered: 2014-09-04

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