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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human LAP(TGF-Beta1) ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human LAP (TGF-Beta1) ELISA Kit PicoKine™ (96 Tests). Quantitate Human LAP(TGF-Beta1) in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LAP(TGF-Beta1) ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1218)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L30-R278
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1218 is reactive to LAP(TGF-Beta1) in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1218 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGFB1
TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.
|EK1218-CAP||Anti-Human LAP(TGF-Beta1) Pre-coated 96-well strip microplate||1|
|EK1218-ST||Human LAP(TGF-Beta1) Standard||2 vials, 10 ng/tube|
|EK1218-DA||Human LAP(TGF-Beta1) Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human LAP(TGF-beta1) PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TGFB1, Dilution ratio of 1:100, concentration in serum is around 80ng/ml.
Some articles we found to cite concentrations of TGFB1 in samples: 18053185 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LAP(TGF-Beta1) ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LAP(TGF-Beta1) (Source: Uniprot.Org, NCBI)
Transforming growth factor beta-1 proprotein
CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LAP(TGF-Beta1), check out the LAP(TGF-Beta1) Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LAP(TGF-Beta1): database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1218 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The TGF‐β/miR‐31/CEACAM1‐S axis inhibits CD4+CD25+ Treg differentiation in systemic lupus erythematosus
Programmable immune activating electrospun fibers for skin regeneration
Inhibitive effect of cordyceps sinensis on experimental hepatic fibrosis and its possible mechanism
HSV-1 infection suppresses TGF-%u03B21 and SMAD3 expression in human corneal epithelial cells
Danggui buxue tang suppresses high glucose-induced proliferation and extracellular matrix accumulation of mesangial cells via inhibiting lncRNA PVT1
Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells
The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice
The effect of positive end-expiratory pressure on inflammatory cytokines during laparoscopic cholecystectomy
MiR-663 inhibits radiation-induced bystander effects by targeting?TGFB1?in a feedback mode
Santagata S, Napolitano M, D'Alterio C, Desicato S, Maro SD, Marinelli L, Fragale A, Buoncervello M, Persico F, Gabriele L, Novellino E, Longo N, Pignata S, Perdon
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