Human LAP(TGF-Beta1) ELISA Kit PicoKine™

Human LAP (TGF-Beta1) ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFB1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 24 publication(s).

Product Info Summary

SKU: EK1218
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human LAP(TGF-Beta1) ELISA Kit PicoKine™

See all TGFB1 products

SKU/Catalog Number

EK1218

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human LAP (TGF-Beta1) ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFB1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human LAP(TGF-Beta1) ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1218)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L30-R278

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1218 is reactive to TGFB1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1218 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1218-CAP Anti-Human TGFB1 Pre-coated 96-well strip microplate 1
EK1218-ST Human TGFB1 Standard 2 vials, 10 ng/tube
EK1218-DA Human TGFB1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0040.0590.1100.2320.4040.8311.4792.261

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TGFB1, Dilution ratio of 1:100, concentration in serum is around 80ng/ml.

Some articles we found to cite concentrations of TGFB1 in samples: 18053185 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human LAP(TGF-Beta1) ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)876732000906982085
Standard deviation3.9143.741264.557.93148.35
CV (%)4.5%6.5%8.3%5%8.3%7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 18710488101957.587.9%
Sample 267368461972167436.515.4%
Sample 320001784201117991898107.25.6%
*number of samples for each test n=16.

Gene/Protein Information For TGFB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01137

Gene ID

7040

Gene Name

TGFB1

Full Name

Transforming growth factor beta-1 proprotein

Weight

44.341kDa

Superfamily

TGF-beta family

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFB1, check out the TGFB1 Infographic

TGFB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1218 has been cited in 24 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibitive effect of cordyceps sinensis on experimental hepatic fibrosis and its possible mechanism

HSV-1 infection suppresses TGF-%u03B21 and SMAD3 expression in human corneal epithelial cells

Danggui buxue tang suppresses high glucose-induced proliferation and extracellular matrix accumulation of mesangial cells via inhibiting lncRNA PVT1

Combination epidermal growth factor receptor variant III peptide-pulsed dendritic cell vaccine with miR-326 results in enhanced killing on EGFRvIII-positive cells

The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice

The effect of positive end-expiratory pressure on inflammatory cytokines during laparoscopic cholecystectomy

MiR-663 inhibits radiation-induced bystander effects by targeting?TGFB1?in a feedback mode

Santagata S, Napolitano M, D'Alterio C, Desicato S, Maro SD, Marinelli L, Fragale A, Buoncervello M, Persico F, Gabriele L, Novellino E, Longo N, Pignata S, Perdon

Denslow A, ?witalska M, Jarosz J, Papiernik D, Porshneva K, Nowak M, Wietrzyk J. PLoS One. 2017 Dec 5;12(12):e0188740. doi: 10.1371/journal.pone.0188740. eCollection 2017. Clopidogrel in a combined therapy with anticancer drugs

Wang Q, Wang J, Wang J, Hong S, Han F, Chen J, Chen G. Mol Med Rep. 2017 May;15(5):3062-3068. doi: 10.3892/mmr.2017.6364. Epub 2017 Mar 23. HMGB1 induces lung fibroblast to myofibroblast differentiation through NF-κB-mediated TGF-β1 release

Bu ZY, Yu XH, Wu LM, Zhong JB, Yang P, Chen J. Exp Ther Med. 2017 Jun;13(6):3327-3332. doi: 10.3892/etm.2017.4418. Epub 2017 May 3. Normalization of regulatory T cells, serum TGF-β1, and LTN after 5-aminolevulinic acid-photodynamic therapy in pati...

Chen Y, Zeng C, Zhan Y, Wang H, Jiang X, Li W. Oncogene. 2017 Aug 17;36(33):4692-4705. doi: 10.1038/onc.2017.100. Epub 2017 Apr 10. Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-media...

Liu, M., Zhen, X., Song, H., Chen, J., Sun, X., Li, X.,..., & Sun, H. (2017). Low-dose lymphocyte immunotherapy rebalances the peripheral blood Th1/Th2/Treg paradigm in patients with unexplained recurrent miscarriage. Reproductive Biology and Endo...

Wenbin Z, Guojun G. West Indian Med J. 2014 Jan;63(1):20-5. Doi: 10.7727/Wimj.2014.008. Epub 2014 Apr 8. Resveratrol Ameliorates Diabetes-Induced Renal Damage Through Regulating The Expression Of Tgf-??1, Collagen Iv And Th17/Treg-Related Cytokine...

Li Wx, Kong X, Zhang Jx, Yang Jr. Food Funct. 2013 Feb 26;4(3):453-60. Doi: 10.1039/C2Fo30220A. Long-Term Intake Of Sesamin Improves Left Ventricular Remodelling In Spontaneously Hypertensive Rats.

Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...

Li B, Yang J, Ma L, Li F, Tu Z, Gao C. J Biomed Mater Res A. 2013 Nov;101(11):3097-108. Doi: 10.1002/Jbm.A.34618. Epub 2013 Mar 25. Fabrication Of Poly(Lactide-Co-Glycolide) Scaffold Filled With Fibrin Gel, Mesenchymal Stem Cells, And Poly(Ethylen...

Sharifi N, Amani R, Hajiani E, Cheraghian B. Endocrine. 2014 Sep;47(1):70-80. Doi: 10.1007/S12020-014-0336-5. Epub 2014 Jun 27. Does Vitamin D Improve Liver Enzymes, Oxidative Stress, And Inflammatory Biomarkers In Adults With Non-Alcoholic Fatty ...

Huang Xq, Zhang Xy, Wang Xr, Yu Sy, Fang Sh, Lu Yb, Zhang Wp, Wei Eq. J Neuroinflammation. 2012 Jun 26;9:145. Doi: 10.1186/1742-2094-9-145. Transforming Growth Factor ??1-Induced Astrocyte Migration Is Mediated In Part By Activating 5-Lipoxygenase...

Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...

Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...

Shen Ww, Zhao Jh. Bioelectromagnetics. 2010 Feb;31(2):113-9. Doi: 10.1002/Bem.20535. Pulsed Electromagnetic Fields Stimulation Affects Bmd And Local Factor Production Of Rats With Disuse Osteoporosis.

Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.

Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.

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8 Customer Q&As for Human LAP(TGF-Beta1) ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated TGFB1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGFB1 antigen?

B. Scott

Verified customer

Asked: 2020-10-05

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-10-05

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TGFB1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

C. Yang

Verified customer

Asked: 2020-01-22

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-01-22

Question

Q: is it okay to use citrate plasma as samples in Human TGFB1 Picokine® ELISA Kit (Catalog # EK1218)?

K. Hernandez

Verified customer

Asked: 2019-07-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TGFB1 causing disruption of its protein structure. TGFB1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the TGFB1 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-07-13

Question

Q: Can TGFB1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-02-25

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-02-25

Question

Q: What is the optimal O.D. value for TGFB1 ELISA kit? I performed your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TGFB1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-03-29

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

R. Chakrabarti

Verified customer

Asked: 2017-10-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-10-26

Question

Q: how to proceed with the analysis of ELISA data? I measured TGFB1 level in serum.

M. Wu

Verified customer

Asked: 2017-09-15

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-09-15

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2014-05-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-05-10

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