Overview
| Product Name |
Human LOX-1/OLR1 ELISA Kit PicoKine™
See more LOX-1/OLR1 products |
| Catalog# |
EK0824 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Human LOX-1/OLR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human OLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human LOX-1/OLR1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0824)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: S61-Q273 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For OLR1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P78380 |
|---|
| NCBI Gene Id | 4973 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | OLR1 |
|---|
| Protein Name | Oxidized low-density lipoprotein receptor 1 |
|---|
| Alternative Names | LOX-1/OLR1|CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1 |
|---|
| Molecular Weight | 30959 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on OLR1, check out the OLR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for OLR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the OLR1 infographic
Kit Components And Assay QC Data Details Of Human LOX-1/OLR1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0824-CAP |
Anti-Human OLR1 Pre-coated 96-well strip microplate | 1 |
| EK0824-ST | Human OLR1 Standard | 2 vials, 10 ng/tube |
| EK0824-DA | Human OLR1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0824 Human LOX-1/OLR1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.030 | 0.162 | 0.219 | 0.384 | 0.694 | 1.198 | 1.694 | 2.032 |
We measured random samples of Human LOX-1/OLR1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 88 | 211 | 833 | 90 | 192 | 784 |
| Standard deviation | 6.86 | 12.02 | 64.14 | 8.46 | 10.94 | 69.77 |
| CV (%) | 7.8% | 5.7% | 7.7% | 9.4% | 5.7% | 8.9% |
We measured the performance consistency of Human LOX-1/OLR1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 88 | 77 | 81 | 83 | 82 | 3.96 | 4.8% |
| Sample 2 | 211 | 220 | 195 | 196 | 205 | 10.5 | 5.1% |
| Sample 3 | 833 | 881 | 979 | 973 | 916 | 61.9 | 6.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Total number of citations: 1
| Polyphenol- and Caffeine-Rich Postfermented Pu-erh Tea Improves Diet-Induced Metabolic Syndrome by Remodeling Intestinal Homeostasis in Mice |
PubMed ID 29061705 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0824 Human LOX-1/OLR1 ELISA Kit PicoKine™
8 Related Questions
Question
Is it possible to detect/determine the soluble for of LOX-1 with this kit, EK0824?
Verified Customer
Asked: 2020-04-28
Answer
EK0824 is able to detect the soluble form of LOX-1.
Boster Scientific Support
Answered: 2020-04-28
Question
Thank you very much for answering our question quickly.
Verified Customer
Asked: 2019-09-26
Answer
Please see the following suggested dilution ratios for
Boster Scientific Support
Answered: 2019-09-26
Question
Can EK0824 detect the soluble form of LOX-1?
Verified Customer
Asked: 2014-03-28
Answer
EK0824 can detect the soluble form of LOX-1.
Boster Scientific Support
Answered: 2014-03-28
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of LOX-1 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Asked: 2020-03-04
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LOX-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement LOX-1 using the EK0824 Human LOX-1 Picokine® ELISA Kit?
Verified Customer
Asked: 2020-02-23
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-02-23
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Edwards
Asked: 2019-04-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-04
Question
Q: if the enzyme conjugated LOX-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LOX-1 antigen?
C. Patel
Asked: 2018-09-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-11
Question
Q: how to analyze ELISA data? I have obtained LOX-1 level in plasma.
T. Kaur
Asked: 2016-12-20
Boster Scientific Support
Answered: 2016-12-20
