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Product Info Summary
| SKU: | EK0824 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human LOX-1/OLR1 ELISA Kit PicoKine®
View all LOX-1/OLR1 ELISA kits
SKU/Catalog Number
EK0824
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LOX-1/OLR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human OLR1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LOX-1/OLR1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0824)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S61-Q273
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0824 is reactive to OLR1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0824 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LOX-1/OLR1
LOX-1 (lectin-type oxidized LDL receptor 1) also known as OLR1, is a protein that in humans is encoded by the OLR1 gene. LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. The LOX1 gene is mapped to 12p13-p12 by fluorescence in situ hybridization. LOX1 is expressed on the plasma membrane of differentiated macrophages, but not on monocytes. The LOX1 protein acts as a macrophage scavenger receptor. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0824-CAP | Anti-Human OLR1 Pre-coated 96-well strip microplate | 1 |
| EK0824-ST | Human OLR1 Standard | 2 vials, 10 ng/tube |
| EK0824-DA | Human OLR1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.030 | 0.162 | 0.219 | 0.384 | 0.694 | 1.198 | 1.694 | 2.032 |
Data Example Images
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Human LOX-1/OLR1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LOX-1/OLR1, Dilution ratio of 1:1, concentration in serum is around 300pg/ml.
Some articles we found to cite concentrations of LOX-1/OLR1 in samples: 21364202 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LOX-1/OLR1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 88 | 211 | 833 | 90 | 192 | 784 |
| Standard deviation | 6.86 | 12.02 | 64.14 | 8.46 | 10.94 | 69.77 |
| CV (%) | 7.8% | 5.7% | 7.7% | 9.4% | 5.7% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of LOX-1/OLR1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 88 | 77 | 81 | 83 | 82 | 3.96 | 4.8% |
| Sample 2 | 211 | 220 | 195 | 196 | 205 | 10.5 | 5.1% |
| Sample 3 | 833 | 881 | 979 | 973 | 916 | 61.9 | 6.7% |
Protein Target Info & Infographic
Gene/Protein Information For OLR1 (Source: Uniprot.Org, NCBI)
Uniprot ID
P78380
Gene ID
4973
Gene Name
OLR1
Full Name
Oxidized low-density lipoprotein receptor 1
Weight
30.959kDa
Alternative Names
CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1 OLR1 CLEC8A, LOX1, LOXIN, SCARE1, SLOX1 oxidized low density lipoprotein receptor 1 oxidized low-density lipoprotein receptor 1|C-type lectin domain family 8 member A|hLOX-1|lectin-type oxidized LDL receptor 1|ox LDL receptor 1|oxidized low density lipoprotein (lectin-like) receptor 1|oxidized low-density lipoprotein receptor 1, soluble form|scavenger receptor class E, member 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on OLR1, check out the OLR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for OLR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human LOX-1/OLR1 ELISA Kit PicoKine® (EK0824)
EK0824 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
8 Customer Q&As for Human LOX-1/OLR1 ELISA Kit PicoKine®
Question
Is it possible to detect/determine the soluble for of LOX-1 with this kit, EK0824?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
EK0824 is able to detect the soluble form of LOX-1.
Boster Scientific Support
Answered: 2020-04-28
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of LOX-1 in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LOX-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement LOX-1 using the EK0824 Human LOX-1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-23
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-02-23
Question
Thank you very much for answering our question quickly.
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
Please see the following suggested dilution ratios for
Boster Scientific Support
Answered: 2019-09-26
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
S. Edwards
Verified customer
Asked: 2019-04-04
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-04-04
Question
Q: if the enzyme conjugated LOX-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the LOX-1 antigen?
C. Patel
Verified customer
Asked: 2018-09-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-11
Question
Q: how to analyze ELISA data? I have obtained LOX-1 level in plasma.
T. Kaur
Verified customer
Asked: 2016-12-20
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-12-20
Question
Can EK0824 detect the soluble form of LOX-1?
Verified Customer
Verified customer
Asked: 2014-03-28
Answer
EK0824 can detect the soluble form of LOX-1.
Boster Scientific Support
Answered: 2014-03-28
