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Human LOX-1/OLR1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human LOX-1/OLR1. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human LOX-1/OLR1 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0824 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 31.2pg/ml-2000pg/ml |
Overview
| Product Name | Human LOX-1/OLR1 ELISA Kit PicoKine™
See all LOX-1/OLR1 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0824 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Lox1/OLR1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Lox1/OLR1 tissue specificity: Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. . |
| Cite This Product | Human LOX-1/OLR1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0824) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 31.2pg/ml-2000pg/ml |
| Immunogen/Standard | Expression system for standard: NSO; Immunogen sequence: S61-Q273 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Gene/Protein Basic Information For OLR1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P78380 |
|---|---|
| NCBI Gene Id | 4973 |
| Species Of This Entry | Human |
| Gene Name | OLR1 |
| Protein Name | Oxidized low-density lipoprotein receptor 1 |
| Alternative Names | LOX-1/OLR1|CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1 |
| Molecular Weight | 30959 |
| Post Tranlational Modifications | Phosphorylation; Cleavage; Oxidation; Glycosylation; Acetylation; Reduction; Dephosphorylation; Methylation; Nitrosylation; Carbamylation; Prenylation; Iodination; Farnesylation |
| Subcellular Localization | Cell membrane; Lipid-anchor. Cell membrane; Single-pass type II membrane protein. Membrane raft. Secreted. A secreted form also exists. Localization to membrane rafts requires palmitoylation. |
| Gene Location | 12p13.2, on Chromasome 12, gene sequence: NC_000012.12 (10158300..10172191, complement) |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For OLR1 (Source: Uniprot.org, NCBI)
| Protein Function | Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. |
|---|---|
| Tissue Specificity | Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level. |
| Interacting Proteins | CCT4; TCP1 |
| Related Diseases | Atherosclerosis; Tissue Adhesions; Arteriosclerosis; Inflammation; Cardiovascular Diseases; Dental Plaque; Coronary Artery Disease; Hypertensive Disease; Diabetes Mellitus; Myocardial Infarction; Infarction; Vascular Diseases; Hypercholesterolemia; Alzheimer's Disease; Obesity; Coronary Heart Disease; Hyperlipidemia; Acute Coronary Syndrome |
| Related Pathways | Pathogenesis; Endocytosis; Secretion; Cell Adhesion; Cell Death; Cell Proliferation; Phagocytosis; Localization; Immune Response; Inflammatory Response; Angiogenesis; Autophagy; Tube Formation; Dna Methylation; Glycosylation; Transport; Aging; Fertilization; Transcytosis; Reverse Transcription |
| Background | LOX-1(lectin-type oxidized LDL receptor 1) also known as OLR1, is a protein that in humans is encoded by the OLR1 gene. LOX-1 is a receptor protein which belongs to the C-type lectin superfamily. The LOX1 gene is mapped to 12p13-p12 by fluorescence in situ hybridization. LOX1 is expressed on the plasma membrane of differentiated macrophages, but not on monocytes. The LOX1 protein acts as a macrophage scavenger receptor. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD. |
| Scientific References | PMID: 9052782 by Sawamura T., et al. An endothelial receptor for oxidized low-density lipoprotein. PMID: 9763655 by Li X., et al. Assignment of the human oxidized low-density lipoprotein receptor gene (OLR1) to chromosome 12p13.1-->p12.3, and identification of a polymorphic CA-repeat marker in the OLR1 gene. |
Kit Components And Assay QC Data Details Of Human LOX-1/OLR1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0824-CAP | 96-well plate precoated with anti-Human LOX-1/OLR1 antibody | 1 |
| EK0824-ST | lyophilized recombinant Human LOX-1/OLR1 standard | 10ng/tube |
| EK0824-DA | biotinylated anti-Human LOX-1/OLR1 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0824 Human LOX-1/OLR1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.030 | 0.162 | 0.219 | 0.384 | 0.694 | 1.198 | 1.694 | 2.032 |
We measured random samples of Human LOX-1/OLR1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 88 | 211 | 833 | 90 | 192 | 784 |
| Standard deviation | 6.86 | 12.02 | 64.14 | 8.46 | 10.94 | 69.77 |
| CV(%) | 7.8% | 5.7% | 7.7% | 9.4% | 5.7% | 8.9% |
We measured the performance consistency of Human LOX-1/OLR1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 88 | 77 | 81 | 83 | 82 | 3.96 | 4.8% |
| Sample 2 | 211 | 220 | 195 | 196 | 205 | 10.5 | 5.1% |
| Sample 3 | 833 | 881 | 979 | 973 | 916 | 61.9 | 6.7% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all LOX-1/OLR1 primary antibodies,Other Recommended Resources
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Total number of citations: 1
| Polyphenol- and Caffeine-Rich Postfermented Pu-erh Tea Improves Diet-Induced Metabolic Syndrome by Remodeling Intestinal Homeostasis in Mice | PubMed ID 29061705 |
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