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Product Info Summary
| SKU: | EK2238 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human LPL ELISA Kit PicoKine®
SKU/Catalog Number
EK2238
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LPL PicoKine® ELISA Kit (96 Tests). Quantitate Human LPL in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 40 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LPL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2238)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: A28-G475
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<40 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2238 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2238 is reactive to LPL in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2238 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LPL
LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2238-CAP | Anti-Human LPL Pre-coated 96-well strip microplate | 1 |
| EK2238-ST | Human LPL Standard | 2 vials, 10 ng/tube |
| EK2238-DA | Human LPL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.073 | 0.167 | 0.225 | 0.369 | 0.6259 | 1.107 | 1.793 | 2.4 |
Data Example Images
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Human LPL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LPL, dilution ratio of 1:1, concentration in serum and plasma is around 900 pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LPL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 137 | 201 | 2403 | 127 | 163 | 2010 |
| Standard deviation | 9.55 | 9.27 | 160.76 | 5.82 | 11.8 | 98.49 |
| CV(%) | 7% | 4.6% | 6.7% | 4.6% | 7.2% | 4.9% |
Specific Publications For Human LPL ELISA Kit PicoKine® (EK2238)
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