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Product Info Summary
| SKU: | EK1479 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Human Marapsin/Pancresin ELISA Kit PicoKine®
View all Marapsin/Pancreasin ELISA kits
SKU/Catalog Number
EK1479
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Marapsin/Pancresin ELISA Kit PicoKine™ (96 Tests). Quantitate Human PRSS27 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Marapsin/Pancresin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1479)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M35-K290
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1479 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1479 is reactive to PRSS27 in Human samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK1479 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Marapsin/Pancreasin
Pancreasin, also known as PRSS27 or Marapsin, is a pancreatic tryptic serine peptidase that cleaves peptides after an arginine residue. It belongs to the group-1 subfamily of serine proteases. The pancreasin gene is mapped to chromosome 16p13.3. Pancreasin is resistant to inactivation by large molecular mass trypsin inhibitors, but it is sensitive to benzamidine and leupeptin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1479-CAP | Anti-Human PRSS27 Pre-coated 96-well strip microplate | 1 |
| EK1479-ST | Human PRSS27 Standard | 2 vials, 10 ng/tube |
| EK1479-DA | Human PRSS27 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 | |
| O.D. | 0.029 | 0.06 | 0.124 | 0.195 | 0.432 | 0.834 | 1.369 | 1.897 |
Data Example Images
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Human Marapsin/Pancresin PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Marapsin/Pancresin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 167 | 13161 | 5662 | 161 | 1417 | 5245 |
| Standard deviation | 10.35 | 58.59 | 311.41 | 12.39 | 77.93 | 326.2%9 |
| CV (%) | 6.2% | 4.2% | 5.5% | 7.7% | 5.5% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Marapsin/Pancreasin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 167 | 176 | 171 | 165 | 169 | 4.2 | 2.4% |
| Sample 2 | 1395 | 1278 | 1352 | 1276 | 1325 | 50.59 | 3.8% |
| Sample 3 | 5662 | 5287 | 5308 | 6068 | 5581 | 318.07 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For PRSS27 (Source: Uniprot.Org, NCBI)
Gene Name
PRSS27
Full Name
Serine protease 27
Weight
31.94kDa
Superfamily
peptidase S1 family
Alternative Names
CAP-2; CAPH2; channel-activating protease 2; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; EC 3.4.21.43; Marapsin; MPN; MPNMarapsin; Pancreasin; protease, serine 27; PRSS27; serine protease 27 PRSS27 CAPH2, MPN serine protease 27 serine protease 27|channel-activating protease 2|marapsin|pancreasin|protease, serine 27
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PRSS27, check out the PRSS27 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PRSS27: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Marapsin/Pancresin ELISA Kit PicoKine® (EK1479)
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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4 Customer Q&As for Human Marapsin/Pancresin ELISA Kit PicoKine®
Question
Q: which procedure should I follow in order to thaw whole blood sample for Marapsin ELISA after freezing?
P. Baker
Verified customer
Asked: 2018-11-24
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Marapsin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-11-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Surve
Verified customer
Asked: 2018-04-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-05
Question
Q: how to analyze ELISA data? I have performed Marapsin level in serum.
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-03-06
Question
Q: if the enzyme conjugated Marapsin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Marapsin antigen?
Verified Customer
Verified customer
Asked: 2016-12-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-09
