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Product Info Summary
| SKU: | M01963 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-S100P Rabbit Monoclonal Antibody
SKU/Catalog Number
M01963
BM4913 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-S100P Rabbit Monoclonal Antibody catalog # M01963. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100P Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01963)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOCH-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S100P
Reactive Species
M01963 is reactive to S100P in Human
Observed Molecular Weight
10 kDa
Calculated molecular weight
10.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01963 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: SW480 cell
IHC: human bladder carcinoma tissue
ICC/IF: SW480 cell
Validation Images & Assay Conditions
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Western blot analysis of S100P expression in SW480 cell lysate.
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Western blot analysis of S100P using anti-S100P antibody (M01963).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100P antigen affinity purified monoclonal antibody (M01963) at 1:500 overnight at 4°C, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for S100P at approximately 10 kDa. The expected band size for S100P is at 10 kDa.
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Immunohistochemical analysis of paraffin-embedded human bladder carcinoma, using S100P Antibody.
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Immunofluorescent analysis of SW480 cells, using S100P Antibody.
Specific Publications For Anti-S100P Rabbit Monoclonal Antibody (M01963)
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4 Customer Q&As for Anti-S100P Rabbit Monoclonal Antibody
Question
We are currently using anti-S100P Rabbit Monoclonal antibody M01963 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
The anti-S100P Rabbit Monoclonal antibody (M01963) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-28
Question
Is a blocking peptide available for product anti-S100P Rabbit Monoclonal antibody (M01963)?
B. Parker
Verified customer
Asked: 2015-10-16
Answer
We do provide the blocking peptide for product anti-S100P Rabbit Monoclonal antibody (M01963). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2015-10-16
Question
Would anti-S100P Rabbit Monoclonal antibody M01963 work on monkey IP with placenta?
A. Anderson
Verified customer
Asked: 2014-12-22
Answer
Our lab technicians have not validated anti-S100P Rabbit Monoclonal antibody M01963 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-S100P Rabbit Monoclonal antibody M01963 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey placenta in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-22
Question
See attached the WB image, lot number and protocol we used for placenta using anti-S100P Rabbit Monoclonal antibody M01963. Please let me know if you require anything else.
K. Wu
Verified customer
Asked: 2013-09-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-30
