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Product Info Summary
| SKU: | EK2243 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA) |
Product info
Product Name
Human MDGA1 ELISA Kit PicoKine®
SKU/Catalog Number
EK2243
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MDGA1 PicoKine ELISA Kit® (96 Tests). Quantitate Human MDGA1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 30 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MDGA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2243)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q19-G931
Sensitivity
<30 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2243 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2243 is reactive to Mdga1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA)
Application Guarantee
EK2243 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Mdga1
This gene encodes a glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that is expressed predominantly in the developing nervous system. In addition to possessing several cell adhesion molecule-like domains, the mature protein has six Ig-like domains, a single fibronectin type III domain, a MAM domain and a C-terminal GPI-anchoring site. Studies in other mammals suggest this protein plays a role in cell adhesion, migration, and axon guidance and, in the developing brain, neuronal migration. In humans, this gene is associated with bipolar disorder and schizophrenia.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2243-CAP | Anti-Human Mdga1 Pre-coated 96-well strip microplate | 1 |
| EK2243-ST | Human Mdga1 Standard | 2 vials, 10 ng/tube |
| EK2243-DA | Human Mdga1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 | 4,000 |
| O.D. | 0.039 | 0.072 | 0.129 | 0.188 | 0.421 | 0.685 | 1.368 | 2.089 |
Data Example Images
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Human MDGA1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MDGA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 71 | 243 | 1519 | 119 | 214 | 1316 |
| Standard deviation | 1.98 | 16.14 | 70.79 | 2.86 | 9.39 | 52.38 |
| CV(%) | 2.8% | 6.6% | 4.7% | 2.4% | 4.4% | 4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Mdga1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 71 | 96 | 76 | 96 | 85 | 4.45 | 5.3% |
| Sample 2 | 243 | 212 | 200 | 153 | 202 | 6.87 | 3.4% |
| Sample 3 | 1519 | 1498 | 1148 | 1690 | 1464 | 58.55 | 4% |
Protein Target Info & Infographic
Gene/Protein Information For Mdga1 (Source: Uniprot.Org, NCBI)
Gene Name
Mdga1
Full Name
MAM domain-containing glycosylphosphatidylinositol anchor protein 1
Weight
Alternative Names
FLJ45018; GPIM; MAM domain containing glycosylphosphatidylinositol anchor 1; MAMDC3; MDGA1 Mdga1|1200011I03Rik, GPIM, Mamdc, Mamdc3|MAM domain containing glycosylphosphatidylinositol anchor 1|MAM domain-containing glycosylphosphatidylinositol anchor protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Mdga1, check out the Mdga1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mdga1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MDGA1 ELISA Kit PicoKine® (EK2243)
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