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Product Info Summary
| SKU: | EK2278 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human MEGF9 ELISA Kit PicoKine®
SKU/Catalog Number
EK2278
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MEGF9 ELISA Kit PicoKine® (96 Tests). Quantitate Human MEGF9 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MEGF9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2278)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A36-N514
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2278 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2278 is reactive to MEGF9 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2278 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MEGF9
MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta‑chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31‑514) plus a 68 aa C‑terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200), followed by five EGF‑like domains (aa 204‑451). MEGF9 may run at approximately 160 kDa in SDS‑PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36‑514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2278-CAP | Anti-Human MEGF9 Pre-coated 96-well strip microplate | 1 |
| EK2278-ST | Human MEGF9 Standard | 2 vials, 10 ng/tube |
| EK2278-DA | Human MEGF9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.046 | 0.161 | 0.258 | 0.432 | 0.663 | 0.987 | 1.361 | 2.056 |
Data Example Images
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Human MEGF9 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MEGF9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 223 | 315 | 3772 | 266 | 463 | 4194 |
| Standard deviation | 12.93 | 19.47 | 117.31 | 5.61 | 36.3 | 249.54 |
| CV(%) | 5.8% | 6.2% | 3.1% | 2.1% | 7.8% | 6.0% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MEGF9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 223 | 215 | 175 | 306 | 230 | 16.96 | 7.4% |
| Sample 2 | 315 | 457 | 345 | 413 | 383 | 26.12 | 6.8% |
| Sample 3 | 3772 | 4291 | 2918 | 4826 | 3952 | 199.17 | 5% |
Protein Target Info & Infographic
Gene/Protein Information For MEGF9 (Source: Uniprot.Org, NCBI)
Gene Name
MEGF9
Full Name
Multiple epidermal growth factor-like domains protein 9
Weight
62.984kDa
Alternative Names
EGFL5; EGFL5KIAA0818; EGF-like protein 5; EGF-like-domain, multiple 5; Epidermal growth factor-like protein 5; MEGF9; Multiple EGF-like domains protein 9; multiple EGF-like-domains 9; multiple epidermal growth factor-like domains protein 9 MEGF9 EGFL5 multiple EGF like domains 9 multiple epidermal growth factor-like domains protein 9|EGF-like-domain, multiple 5|epidermal growth factor-like protein 5
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MEGF9, check out the MEGF9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MEGF9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MEGF9 ELISA Kit PicoKine® (EK2278)
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