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Product Info Summary
| SKU: | EK1552 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human MEP1A ELISA Kit PicoKine®
SKU/Catalog Number
EK1552
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MEP1A ELISA Kit PicoKine® (96 Tests). Quantitate Human MEP1A in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MEP1A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1552)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: V22-Q601
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<50 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1552 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1552 is reactive to MEP1A in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1552 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MEP1A
Meprin A subunit alpha also known as endopeptidase-2 or PABA peptide hydrolase is the alpha subunit of the meprin A enzyme that in humans is encoded by the MEP1A gene. Meprin A subunit alpha, encoded by the **MEP1A** gene, is a component of the meprin A metalloproteinase complex, which is involved in extracellular matrix remodeling and proteolysis. Meprin A is a zinc-dependent endopeptidase found predominantly in the kidneys, intestines, and other tissues. It plays a crucial role in various physiological processes, including inflammation, tissue repair, and the processing of bioactive peptides. The alpha subunit, specifically, is a part of the hetero-oligomeric enzyme complex, which combines with the beta subunit to form an active enzyme. Abnormalities in meprin A function have been associated with several pathological conditions, including kidney diseases and cancer.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1552-CAP | Anti-Human MEP1A Pre-coated 96-well strip microplate | 1 |
| EK1552-ST | Human MEP1A Standard | 2 vials, 100 ng/tube |
| EK1552-DA | Human MEP1A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.026 | 0.114 | 0.189 | 0.32 | 0.605 | 1.111 | 1.812 | 2.522 |
Data Example Images
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Human MEP1A ELISA Kit PicoKine standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MEP1A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 2306 | 10121 | 43453 | 2328 | 7457 | 37974 |
| Standard deviation | 163.03 | 401.8 | 2342.12 | 153.18 | 501.11 | 2529.07 |
| CV(%) | 7.1% | 4% | 5.4% | 6.6% | 6.7% | 6.7% |
Specific Publications For Human MEP1A ELISA Kit PicoKine® (EK1552)
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