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Product Info Summary
| SKU: | EK2279 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human MEPE ELISA Kit PicoKine®
SKU/Catalog Number
EK2279
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MEPE ELISA Kit PicoKine® (96 Tests). Quantitate Human MEPE in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 75 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MEPE ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2279)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: P18-D525
Sensitivity
<75 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2279 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2279 is reactive to MEPE in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK2279 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MEPE/OF45
Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) is a protein that in humans is encoded by the MEPE gene. This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2279-CAP | Anti-Human MEPE Pre-coated 96-well strip microplate | 1 |
| EK2279-ST | Human MEPE Standard | 2 vials, 10 ng/tube |
| EK2279-DA | Human MEPE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.033 | 0.105 | 0.233 | 0.487 | 0.696 | 0.981 | 1.371 | 2.047 |
Data Example Images
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Human MEPE PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MEPE ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 224 | 462 | 4230 | 205 | 609 | 3785 |
| Standard deviation | 15.39 | 18.94 | 157.78 | 15.95 | 19.37 | 123.39 |
| CV(%) | 6.9% | 4.1% | 3.7% | 7.8% | 3.2% | 3.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MEPE/OF45 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 224 | 226 | 307 | 234 | 248 | 18.01 | 7.3% |
| Sample 2 | 462 | 335 | 340 | 457 | 399 | 19.57 | 4.9% |
| Sample 3 | 4230 | 4343 | 3648 | 4613 | 4209 | 247.88 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For MEPE (Source: Uniprot.Org, NCBI)
Gene Name
MEPE
Full Name
Matrix extracellular phosphoglycoprotein
Weight
58.419kDa
Superfamily
PF07175/osteoregulin family
Alternative Names
extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein; MEPE; OF45; Osteoregulin MEPE OF45 matrix extracellular phosphoglycoprotein matrix extracellular phosphoglycoprotein|matrix, extracellular phosphoglycoprotein with ASARM motif (bone)|osteoblast/osteocyte factor 45|osteoregulin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MEPE, check out the MEPE Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MEPE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MEPE ELISA Kit PicoKine® (EK2279)
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