Overview
| Product Name |
Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™
|
| Catalog# |
EK0448 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CCL3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0448)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
15.6 pg/ml - 1,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: A27-A92 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For CCL3 (Source: Uniprot.org, NCBI)
| Uniprot Id | A0N0R1 |
|---|
| Gene Name | CCL3 |
|---|
| Molecular Weight | 10085 MW |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CCL3, check out the CCL3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CCL3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the CCL3 infographic
Kit Components And Assay QC Data Details Of Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0448-CAP |
Anti-Human CCL3 Pre-coated 96-well strip microplate | 1 |
| EK0448-ST | Human CCL3 Standard | 2 vials, 10 ng/tube |
| EK0448-DA | Human CCL3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0448 Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.037 | 0.112 | 0.206 | 0.272 | 0.545 | 0.932 | 1.456 | 2.071 |
We measured random samples of Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 175 | 370 | 30 | 166 | 361 |
| Standard deviation | 2.54 | 10.32 | 14.8 | 2.58 | 12.45 | 20.93 |
| CV (%) | 7.7% | 4% | 4% | 8.6% | 7.5% | 5.8% |
We measured the performance consistency of Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 33 | 34 | 32 | 35 | 33 | 1.11 | 3.3% |
| Sample 2 | 175 | 166 | 172 | 161 | 168 | 5.4 | 3.2% |
| Sample 3 | 370 | 407 | 390 | 394 | 390 | 13.27 | 3.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
Yudan Wang, Xiaomeng Ding, Yi Chen, Jianhua Xie, Bing Zheng, Xinxin Chang, Shiqi Liu, Xiaobo Hu, Qiang Yu,“Dialogue” between Caco-2 and DCs regulated by Ganoderma atrum polysaccharide in intestinal-like Caco-2/DCs co-culture model,Food Research International,2021,110310,ISSN 0963-9969,https://doi.org/10.1016/j.foodres.2021.110310.
Species: Human,Mouse
EK0448 usage in article: APP:ELISA, SAMPLE:CACO2 CELL, DILUTION:NA |
View Publication |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0448 Human MIP-1Alpha/CCL3 ELISA Kit PicoKine™
8 Related Questions
Question
Q: Can CCL3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Asked: 2020-10-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-28
Question
Q: if the enzyme conjugated CCL3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL3 antigen?
W. Jackson
Asked: 2019-08-04
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2018-12-27
Boster Scientific Support
Answered: 2018-12-27
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
G. Maharaj
Asked: 2018-10-04
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-10-04
Question
Q: which procedure should I follow in order to thaw whole blood sample for CCL3 ELISA after freezing?
Verified Customer
Asked: 2018-01-31
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CCL3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-31
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2017-10-28
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-10-28
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CCL3 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CCL3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-02-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2016-04-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-04-19
