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Product Info Summary
| SKU: | EK0467 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human MMP-10/Stromelysin-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0467
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP10 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP10 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-10/Stromelysin-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0467)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Y18-C476
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0467 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0467 is reactive to MMP10 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0467 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-10
Stromelysin-2 also known as matrix metalloproteinase-10 (MMP-10) or transin-2 is an enzyme that in humans is encoded by the MMP10 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0467-CAP | Anti-Human MMP10 Pre-coated 96-well strip microplate | 1 |
| EK0467-ST | Human MMP10 Standard | 2 vials, 10 ng/tube |
| EK0467-DA | Human MMP10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 313 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.038 | 0.149 | 0.215 | 0.349 | 0.564 | 0.961 | 1.66 | 2.024 |
Data Example Images
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Human MMP-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-10, Dilution ratio of 1:1, concentration in serum and plasma is 244-2072 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-10/Stromelysin-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 150 | 657 | 2257 | 156 | 631 | 2147 |
| Standard deviation | 9.75 | 45.33 | 162.5 | 10.76 | 51.11 | 173.9 |
| CV (%) | 6.5% | 6.9% | 7.2% | 6.9% | 8.1% | 8.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-10 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 150 | 142 | 133 | 133 | 139 | 7.08 | 5% |
| Sample 2 | 657 | 650 | 733 | 643 | 670 | 36.27 | 5.4% |
| Sample 3 | 2257 | 2665 | 2447 | 2386 | 2438 | 147.54 | 6% |
Protein Target Info & Infographic
Gene/Protein Information For MMP10 (Source: Uniprot.Org, NCBI)
Gene Name
MMP10
Full Name
Stromelysin-2
Weight
54.151kDa
Superfamily
peptidase M10A family
Alternative Names
EC 3.4.24; EC 3.4.24.22; matrix metallopeptidase 10 (stromelysin 2); matrix metalloprotease 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP10; MMP-10; SL-2; STMY2stromelysin-2; Stromelysin 2; transin 2; transin-2 MMP10 SL-2, STMY2 matrix metallopeptidase 10 stromelysin-2|MMP-10|matrix metalloprotease 10|matrix metalloproteinase 10 (stromelysin 2)|matrix metalloproteinase-10|transin 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP10, check out the MMP10 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MMP-10/Stromelysin-2 ELISA Kit PicoKine® (EK0467)
Hello CJ!
EK0467 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Calcium‑sensing receptor promotes high glucose‑induced myocardial fibrosis via upregulation of the TGF‑β1/Smads pathway in cardiac fibroblasts
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human MMP-10/Stromelysin-2 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Human MMP-10 Picokine® ELISA Kit (Catalog # EK0467)?
Verified Customer
Verified customer
Asked: 2020-12-13
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of MMP-10 causing disruption of its protein structure. MMP-10 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the MMP-10 ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-12-13
Question
Q: how can I thaw whole blood sample for MMP-10 ELISA after freezing?
M. Lee
Verified customer
Asked: 2020-11-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-10 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-11-17
Question
Q: how to proceed with the analysis of ELISA data? I measured MMP-10 level in plasma.
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-04-29
Question
Q: if the enzyme conjugated MMP-10 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-10 antigen?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-06
Question
Q: What is the optimal O.D. value for MMP-10 ELISA kit? I performed your MMP-10 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain MMP-10 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-09-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-10 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-10 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-09-14
Question
Q: Can MMP-10 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-01-26
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-26
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of MMP-10 in Human plasma? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
M. Edwards
Verified customer
Asked: 2018-05-24
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-05-24
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification MMP-10 using the EK0467 Human MMP-10 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-07-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2016-07-06
