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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin) , saliva and urine.|
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Human MMP-7/Matrilysin ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MMP-7 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP7 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Sensitivity: 6pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-7/Matrilysin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0463)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L18-K267
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0463 is reactive to MMP7 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) , saliva and urine.
EK0463 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-7
Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis. Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues. Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas; however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target. The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28KDa. The detected MMP-7 includes zymogen and active enzyme.
|EK0463-CAP||Anti-Human MMP7 Pre-coated 96-well strip microplate||1|
|EK0463-ST||Human MMP7 Standard||2 vials, 10 ng/tube|
|EK0463-DA||Human MMP7 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MMP-7 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-7/Matrilysin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-7 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MMP7 (Source: Uniprot.Org, NCBI)
peptidase M10A family
EC 3.4.24; EC 220.127.116.11; Matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); Matrix metalloproteinase-7; MMP7; MMP-7; MPSL1; Pump-1 protease; PUMP1; PUMP-1; uterine matrilysin; Uterine metalloproteinase MMP7 MMP-7, MPSL1, PUMP-1 matrix metallopeptidase 7 matrilysin|matrin|matrix metallopeptidase 7 (matrilysin, uterine)|matrix metalloproteinase 7 (matrilysin, uterine)|matrix metalloproteinase-7|pump-1 protease|uterine matrilysin|uterine metalloproteinase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP7, check out the MMP7 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0463 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased levels of thymosin β4 in synovial fluid of patients with rheumatoid arthritis: association of thymosin β4 with other factors that are involved in inflammation and bone erosion in joints
CCR10 activation stimulates the invasion and migration of breast cancer cells through the ERK1/2/MMP-7 signaling pathway
Implication of MMP-9 and urokinase plasminogen activator (uPA) in the activation of pro-matrix metalloproteinase (MMP)-13
Zhu, G., Lin, C., Cheng, Z., Wang, Q., Hoffman, R. M., Singh, S. R., ... & Ye, J. (2020). TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140. Cancer Genomics-Proteomics, 17(1), 23-33.
Sun Y, Chen Y, Li S, Lei Y, Xu D, Jiang N, Zhang Y, Cao J, Ke Z. Int J Nanomedicine. 2017 Aug 31;12:6399-6412. doi: 10.2147/IJN.S144033. eCollection 2017. NanoVelcro-captured CTC number concomitant with enhanced serum levels of MMP7 and MMP9 enabl...
Kim Ks, Lee Ya, Choi Hm, Yoo Mc, Yang Hi. Rheumatol Int. 2012 Oct;32(10):3069-75. Epub 2011 Sep 13. Implication Of Mmp-9 And Urokinase Plasminogen Activator (Upa) In The Activation Of Pro-Matrix Metalloproteinase (Mmp)-13.
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