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Product Info Summary
| SKU: | PROTP08476-8 |
|---|---|
| Size: | 5ug,20ug,100ug |
| Origin Species: | Human, Mouse, Rat |
| Source: | Escherichia coli |
| Application: | Cell Culture |
Product info
Product Name
Human/Mouse/Rat recombinant Activin A protein, AF
View all INHBA recombinant proteins
SKU/Catalog Number
PROTP08476-8
Size
5ug,20ug,100ug
Tag
Tag Free
Description
Activin is the member of the TGF beta superfamily of cytokines. The current understanding of Activin A is classified as a hormone, a Growth Factors, and a cytokine. Depending on the different cell type, that overexpression of Activin A can either inhibit or promote cells division. There are important implications for tumor biology. Activin A also increase joint inflammation in rheumatoid arthritis (RA) and induces pathogenic mechanisms in the respiratory system.
Storage & Handling
Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
Cite This Product
Human/Mouse/Rat recombinant Activin A protein, AF (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP08476-8)
Form
Lyophilized
Formulation
The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us.
Purity
>95% as determined by SDS-PAGE.
Molecular weight
The protein has a calculated MW of 13.1 kDa. The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).
Activity
Measure by its ability to inhibit the proliferation of mouse MPC-11 cells. The ED₅₀ for this effect is <10 ng/mL. The specific activity of recombinant human Activin A is approximately >1.0 x 10³ IU/mg.
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Amino Acid Sequence
GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS
Reconstitution
Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 100 μg/mL and incubate the stock solution at room temperature for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
Assay dilution & Images
Validation Images & Assay Conditions
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SDS- PAGE analysis of recombinant human/mouse/rat Activin A
Specific Publications For Human/Mouse/Rat recombinant Activin A protein, AF (PROTP08476-8)
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